Gao Lihua, Zhou Zhengfu, Chen Xiaonan, Zhang Wei, Lin Min, Chen Ming
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Microorganisms. 2020 Mar 23;8(3):451. doi: 10.3390/microorganisms8030451.
is known for its extreme resistance to ionizing radiation, oxidative stress, and other DNA-damaging agents. The robustness of this bacterium primarily originates from its strong oxidative resistance mechanisms. Hundreds of genes have been demonstrated to contribute to oxidative resistance in ; however, the antioxidant mechanisms have not been fully characterized. In this study, comparative proteomics analysis of grown under normal and oxidative stress conditions was conducted using label-free quantitative proteomics. The abundances of 852 of 1700 proteins were found to significantly differ between the two groups. These differential proteins are mainly associated with translation, DNA repair and recombination, response to stresses, transcription, and cell wall organization. Highly upregulated expression was observed for ribosomal proteins such as RplB, Rpsl, RpsR, DNA damage response proteins (DdrA, DdrB), DNA repair proteins (RecN, RecA), and transcriptional regulators (members of TetR, AsnC, and GntR families, DdrI). The functional analysis of proteins in response to oxidative stress is discussed in detail. This study reveals the global protein expression profile of in response to oxidative stress and provides new insights into the regulatory mechanism of oxidative resistance in .
以其对电离辐射、氧化应激和其他DNA损伤剂的极强抗性而闻名。这种细菌的稳健性主要源于其强大的抗氧化机制。数百个基因已被证明有助于抗氧化；然而，抗氧化机制尚未完全阐明。在本研究中，使用无标记定量蛋白质组学对在正常和氧化应激条件下生长的[细菌名称未给出]进行了比较蛋白质组学分析。发现1700种蛋白质中的852种在两组之间丰度有显著差异。这些差异蛋白质主要与翻译、DNA修复和重组、应激反应、转录以及细胞壁组织相关。观察到核糖体蛋白如RplB、Rpsl、RpsR、DNA损伤反应蛋白（DdrA、DdrB）、DNA修复蛋白（RecN、RecA）以及转录调节因子（TetR、AsnC和GntR家族成员、DdrI）的表达高度上调。详细讨论了蛋白质对氧化应激的功能分析。本研究揭示了[细菌名称未给出]在氧化应激下的全局蛋白质表达谱，并为[细菌名称未给出]的抗氧化调控机制提供了新的见解。
