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来自生沃氏菌 R12 的 Dps DNA 结合蛋白的序列、结构和功能。

Sequence, structure, and function of the Dps DNA-binding protein from Deinococcus wulumuqiensis R12.

机构信息

College of Food Science and Light Industry, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China.

出版信息

Microb Cell Fact. 2022 Jul 2;21(1):132. doi: 10.1186/s12934-022-01857-7.

DOI:10.1186/s12934-022-01857-7
PMID:35780107
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9250271/
Abstract

Deinococcus wulumuqiensis R12, which was isolated from arid irradiated soil in Xinjiang province of China, belongs to a genus that is well-known for its extreme resistance to ionizing radiation and oxidative stress. The DNA-binding protein Dps has been studied for its great contribution to oxidative resistance. To explore the role of Dps in D. wulumuqiensis R12, the Dps sequence and homology-modeled structure were analyzed. In addition, the dps gene was knocked out and proteomics was used to verify the functions of Dps in D. wulumuqiensis R12. Docking data and DNA binding experiments in vitro showed that the R12 Dps protein has a better DNA binding ability than the Dps1 protein from D. radiodurans R1. When the dps gene was deleted in D. wulumuqiensis R12, its resistance to HO and UV rays was greatly reduced, and the cell envelope was destroyed by HO treatment. Additionally, the qRT-PCR and proteomics data suggested that when the dps gene was deleted, the catalase gene was significantly down-regulated. The proteomics data indicated that the metabolism, transport and oxidation-reduction processes of D. wulumuqiensis R12 were down-regulated after the deletion of the dps gene. Overall, the data conformed that Dps protein plays an important role in D. wulumuqiensis R12.

摘要

从中国新疆干旱辐照土壤中分离到的极端耐辐射球菌 R12 属于一种以对电离辐射和氧化应激的极端抗性而闻名的属。DNA 结合蛋白 Dps 因其对氧化抗性的巨大贡献而被研究。为了探索 Dps 在极端耐辐射球菌 R12 中的作用，分析了 Dps 序列和同源建模结构。此外，敲除 dps 基因并用蛋白质组学验证了 Dps 在 R12 中的功能。对接数据和体外 DNA 结合实验表明，R12 的 Dps 蛋白比来自 D. radiodurans R1 的 Dps1 蛋白具有更好的 DNA 结合能力。当 dps 基因在极端耐辐射球菌 R12 中被删除时，其对 HO 和 UV 射线的抵抗力大大降低，并且细胞包膜在 HO 处理下被破坏。此外，qRT-PCR 和蛋白质组学数据表明，当 dps 基因缺失时，过氧化氢酶基因显著下调。蛋白质组学数据表明，删除 dps 基因后，极端耐辐射球菌 R12 的代谢、运输和氧化还原过程下调。总体而言，数据表明 Dps 蛋白在极端耐辐射球菌 R12 中发挥着重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/4ddfe07511c2/12934_2022_1857_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/8d124b091bed/12934_2022_1857_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/b07b408b98c4/12934_2022_1857_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/636f9a024961/12934_2022_1857_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/086cf29a4aeb/12934_2022_1857_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/3ed4eac4cf45/12934_2022_1857_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/a1a33c638211/12934_2022_1857_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/aec03818057c/12934_2022_1857_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/bd3a778310d8/12934_2022_1857_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/4ddfe07511c2/12934_2022_1857_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/8d124b091bed/12934_2022_1857_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/b07b408b98c4/12934_2022_1857_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/636f9a024961/12934_2022_1857_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/086cf29a4aeb/12934_2022_1857_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/3ed4eac4cf45/12934_2022_1857_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/a1a33c638211/12934_2022_1857_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/aec03818057c/12934_2022_1857_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/bd3a778310d8/12934_2022_1857_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9845/9250271/4ddfe07511c2/12934_2022_1857_Fig9_HTML.jpg

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