Department of Chemistry, Shanghai Stomatological Hospital, Fudan University, Shanghai, China.
Changhai Hospital, The Naval Military Medical University, Shanghai, China.
NPJ Biofilms Microbiomes. 2020 Mar 24;6(1):14. doi: 10.1038/s41522-020-0123-4.
Pathogenesis of colorectal cancer (CRC) is associated with alterations in gut microbiome. Previous studies have focused on the changes of taxonomic abundances by metagenomics. Variations of the function of intestinal bacteria in CRC patients compared to healthy crowds remain largely unknown. Here we collected fecal samples from CRC patients and healthy volunteers and characterized their microbiome using quantitative metaproteomic method. We have identified and quantified 91,902 peptides, 30,062 gut microbial protein groups, and 195 genera of microbes. Among the proteins, 341 were found significantly different in abundance between the CRC patients and the healthy volunteers. Microbial proteins related to iron intake/transport; oxidative stress; and DNA replication, recombination, and repair were significantly alternated in abundance as a result of high local concentration of iron and high oxidative stress in the large intestine of CRC patients. Our study shows that metaproteomics can provide functional information on intestinal microflora that is of great value for pathogenesis research, and can help guide clinical diagnosis in the future.
结直肠癌（CRC）的发病机制与肠道微生物组的改变有关。以前的研究主要集中在宏基因组学上分类丰度的变化。与健康人群相比，CRC 患者肠道细菌功能的变化在很大程度上仍然未知。在这里，我们收集了 CRC 患者和健康志愿者的粪便样本，并使用定量代谢组学方法对其微生物组进行了表征。我们已经鉴定和定量了 91902 种肽、30062 种肠道微生物蛋白组和 195 种微生物属。在这些蛋白质中，有 341 种在 CRC 患者和健康志愿者中的丰度存在显著差异。由于 CRC 患者大肠中局部铁浓度高和氧化应激水平高，与铁摄入/转运、氧化应激和 DNA 复制、重组和修复相关的微生物蛋白的丰度发生了显著变化。我们的研究表明，代谢组学可以为发病机制研究提供有关肠道微生物群的功能信息，并且将来有助于指导临床诊断。
