Kennedy M J, Rosnick D K, Ulrich R G, Yancey R J
Upjohn Company, Kalamazoo, Michigan 49001.
J Gen Microbiol. 1988 Jun;134(6):1565-76. doi: 10.1099/00221287-134-6-1565.
The association of Treponema hyodysenteriae with porcine caecal and colonic mucosal surfaces was studied by electron microscopy after orogastric inoculation of pigs with pure cultures. Examination of caecal and colonic mucosa from infected and control animals revealed that large numbers of the spirochaete were associated only with intestinal mucosal surfaces of infected animals. Further examination of the intestinal mucosa from infected pigs showed that T. hyodysenteriae colonized two sites preferentially: the mucus-filled crypts of Lieberkühn and the mucus gel covering the epithelium. Furthermore, no evidence of either specific or nonspecific adhesion to the epithelium proper was found, suggesting that penetration of, or trapping in the mucus gel may be the predominant mechanism of mucosal association by T. hyodysenteriae. Moreover, T. hyodysenteriae was also observed to be highly motile in intestinal mucus, moving faster than any other organism present, and this 'high speed' motility appeared to facilitate penetration into the mucosa. The pattern of motility observed was also highly suggestive of chemotaxis, and this was subsequently confirmed using an in vitro assay to porcine mucus material. It is suggested, therefore, that motility and chemotaxis are important factors/mechanisms in the association and colonization of porcine intestinal mucosa by T. hyodysenteriae.
在用纯培养物经口胃接种猪后，通过电子显微镜研究了猪痢疾密螺旋体与猪盲肠和结肠黏膜表面的关联。对感染动物和对照动物的盲肠和结肠黏膜进行检查发现，大量螺旋体仅与感染动物的肠黏膜表面相关联。对感染猪的肠黏膜进一步检查表明，猪痢疾密螺旋体优先定殖于两个部位：利伯kühn氏黏液填充隐窝和覆盖上皮的黏液凝胶。此外，未发现对上皮本身有特异性或非特异性黏附的证据，这表明在黏液凝胶中穿透或滞留可能是猪痢疾密螺旋体与黏膜关联的主要机制。此外，还观察到猪痢疾密螺旋体在肠黏液中具有高度运动性，其移动速度比存在的任何其他生物体都快，并且这种“高速”运动性似乎有助于其穿透黏膜。观察到的运动模式也强烈暗示了趋化性，随后使用针对猪黏液物质的体外试验证实了这一点。因此，有人提出，运动性和趋化性是猪痢疾密螺旋体与猪肠黏膜关联和定殖的重要因素/机制。
