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由两个不同物种的细胞碎片重构而成的细胞会增殖并形成菌落。

Cells reconstituted from cell fragments of two different species multiply and form colonies.

作者信息

Krondahl U, Bols N, Ege T, Linder S, Ringertz N R

出版信息

Proc Natl Acad Sci U S A. 1977 Feb;74(2):606-9. doi: 10.1073/pnas.74.2.606.

Abstract

Nuclei (minicells) prepared by cytochalasin-induced enucleation of rat myoblasts (L6) were fused with inactivated Sendai virus to preparations of cytoplasms from mouse fibroblasts (A9). Cell colonies arising from such fusion mixtures were examined 5 days after fusion. In the presence of virus a new type of colony was obtained. The cells in these colonies were derived from reconstituted cells capable of undergoing repeated cell divisions.

摘要

通过细胞松弛素诱导大鼠成肌细胞(L6)去核制备的细胞核(微细胞),与仙台病毒灭活后与小鼠成纤维细胞(A9)的细胞质制剂融合。融合混合物产生的细胞集落于融合后5天进行检查。在病毒存在的情况下,获得了一种新型集落。这些集落中的细胞来源于能够进行反复细胞分裂的重构细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0db9/392340/1f6c9574bcc5/pnas00024-0221-a.jpg

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