State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Sci Total Environ. 2020 Jun 20;722:137171. doi: 10.1016/j.scitotenv.2020.137171. Epub 2020 Feb 24.
Hexaconazole is a triazole fungicide, which is stable and difficult to degrade in the environment. The existence of hexaconazole could pose a certain risk to the environment and the health of living organisms. In this study, an efficient degradation strain B-3 (Sphingobacterium multivorum) of hexaconazole was isolated from sewage, activated sludge, and soil. The degradation efficiency of hexaconazole can reach 85.6% in 6 days at a temperature of 32.5 °C, pH of 6.31, initial inoculum of 0.4 g L and initial concentration of hexaconazole of 50 mg L. During degradation, three metabolites (M1: 2-(2, 4-dichlorophenyl)-1-(1H-1, 2, 4-triazol-1-yl) hexane-2, 5-diol; M2: 2-(2, 4-dichlorophenyl) hexane-1, 2-diol; M3: 1H-1, 2, 4-triazole) were identified. Moreover, 45.6% hexaconazole can be degraded in 60 days in natural soil containing B-3. The results of the transcriptome sequencing indicated the presence of 864 differential genes, in which aldehyde dehydrogenase, monooxygenase, RND transporters, and ABC transporters were up-regulated. The generation of 2-(2, 4-dichlorophenyl)-1-(1H-1, 2, 4-triazol-1-yl) hexane-2, 5-diol may be due to the participation of monooxygenase.
己唑醇是一种三唑类杀菌剂，在环境中稳定且难以降解。己唑醇的存在可能对环境和生物机体的健康构成一定风险。本研究从污水、活性污泥和土壤中分离出一株高效降解己唑醇的菌株 B-3（鞘氨醇单胞菌）。在 32.5℃、pH 值为 6.31、初始接种量为 0.4 g·L-1 和己唑醇初始浓度为 50 mg·L-1 的条件下，该菌株在 6 天内可将己唑醇的降解率达到 85.6%。在降解过程中，共鉴定出 3 种代谢产物（M1：2-（2,4-二氯苯基）-1-（1H-1,2,4-三唑-1-基）己烷-2,5-二醇；M2：2-（2,4-二氯苯基）己烷-1,2-二醇；M3：1H-1,2,4-三唑）。此外，在含有 B-3 的天然土壤中，60 天内可降解 45.6%的己唑醇。转录组测序结果表明，共存在 864 个差异基因，其中醛脱氢酶、单加氧酶、RND 转运蛋白和 ABC 转运蛋白被上调。2-（2,4-二氯苯基）-1-（1H-1,2,4-三唑-1-基）己烷-2,5-二醇的生成可能与单加氧酶的参与有关。
