Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, Brazil.
Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, Brazil,
Caries Res. 2020;54(2):185-193. doi: 10.1159/000506439. Epub 2020 Mar 26.
The relationship between the concentration of chemically soluble fluoride found in toothpaste and that present in saliva, during and after brushing, was evaluated as an indicator of potentially bioavailable fluoride in toothpaste. Ten adult participants brushed their teeth with the assigned toothpastes: group I: fresh sample of a fluoride toothpaste: Na2FPO3/CaCO3, 1,378 µg F/g of total soluble fluoride (TSF); groups II-IV: aged samples of toothpaste presenting TSF concentrations of 1,160, 900, and 597 µg F/g, respectively; group V: non-F placebo toothpaste. The volunteers brushed their teeth for 1 min with 0.7 g of the toothpaste, all toothbrushing residues (TR) produced were collected, the mouth was rinsed with water, and saliva samples were collected up to 120 min. Total fluoride (TF) and TSF concentrations were determined in TR and in saliva samples using a fluoride ion-selective electrode. TSF concentration (µg F/mL) in TR was determined as an indicator of fluoride bioavailability during toothbrushing and the areas under curves of saliva fluoride concentration versus time (area under the curve, AUC = µg F/mL × min) were calculated as an indicator of fluoride bioavailability after toothbrushing. A significant correlation was found between the TSF concentrations in the toothpastes and the variables TR (r = 0.850; p = 0.0001) and AUC (r = 0.445; p = 0.004). For TF no significant correlation was found for TR (r = -0.099; p = 0.542) and AUC (r = -0.018; p = 0.912). The findings suggest that TSF concentration chemically found in Na2FPO3/CaCO3-based toothpaste could estimate how much fluoride would be bioavailable in saliva when the teeth are brushed.
研究评估了牙膏中可溶氟化物浓度与刷牙过程中和刷牙后唾液中氟化物浓度的关系，以此作为牙膏中潜在可利用氟化物的指标。10 名成年参与者使用指定的牙膏刷牙：I 组：含氟牙膏的新鲜样本：Na2FPO3/CaCO3，总可溶性氟化物（TSF）中的氟含量为 1,378μg/g；II-IV 组：分别为 TSF 浓度为 1,160μg/g、900μg/g 和 597μg/g 的陈旧牙膏样本；V 组：非含氟安慰剂牙膏。志愿者用 0.7g 牙膏刷牙 1 分钟，收集所有刷牙残留物（TR），用清水漱口，并在 120 分钟内收集唾液样本。使用氟离子选择性电极测定 TR 和唾液样本中的总氟（TF）和 TSF 浓度。TR 中的 TSF 浓度（μg F/mL）被视为刷牙过程中氟化物生物利用度的指标，计算唾液氟化物浓度随时间的曲线下面积（AUC=μg F/mL×min）作为刷牙后氟化物生物利用度的指标。牙膏中的 TSF 浓度与 TR（r=0.850；p=0.0001）和 AUC（r=0.445；p=0.004）变量呈显著相关。对于 TF，TR（r=-0.099；p=0.542）和 AUC（r=-0.018；p=0.912）均未发现显著相关性。研究结果表明，Na2FPO3/CaCO3 基牙膏中化学检测到的 TSF 浓度可用于估计刷牙时唾液中氟化物的生物利用度。
