Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico.
Universidad Juárez Autónoma de Tabasco, Carretera Cunduacán-Jalpa Km. 0.5, Cunduacán 86690, Tabasco, Mexico.
Molecules. 2020 Mar 24;25(6):1454. doi: 10.3390/molecules25061454.
L. is employed for the treatment of pain and as an antidote against the poison of venomous animals in traditional medicine. However, reports have not been found, to our knowledge, about the evaluation of the antinociceptive activity of extracts nor about the presence of compounds associated with this activity. Thus, the purpose of this work was to evaluate the antinociceptive activity of extracts and compounds isolated from the stems of L. The extracts were obtained with solvents of increasing polarity and the compounds were isolated and characterized by column chromatography, HPLC, and NMR. The antinociceptive activity was carried out by the formalin test in mice. Ethyl acetate (AoEA) and methanolic (AoM) extracts decreased the paw licking in both phases of the formalin test. The isolated compounds (kaurenoic acid and hinokinin) from AoEA showed the highest antinociceptive activity in both phases of the formalin test. These results confirmed the analgesic effect of this specie described in traditional medicine and provided a base for a novel analgesic agent. They also allowed an approach for the development of standardized plant extracts with isolated metabolites.
L. 被用于传统医学中的止痛和抗毒蛇毒液中毒。然而，据我们所知，尚未有关于提取物的镇痛活性评估或与该活性相关的化合物存在的报告。因此，本工作的目的是评估从 L. 的茎中分离得到的提取物和化合物的镇痛活性。提取物是用极性递增的溶剂获得的，化合物则通过柱层析、HPLC 和 NMR 进行分离和表征。镇痛活性通过小鼠福尔马林试验进行评估。乙酸乙酯（AoEA）和甲醇（AoM）提取物减少了福尔马林试验的两个阶段中的舔足行为。从 AoEA 中分离得到的化合物（贝壳杉烯酸和扁柏酚）在福尔马林试验的两个阶段中表现出最高的镇痛活性。这些结果证实了该物种在传统医学中描述的镇痛作用，并为新型镇痛剂提供了基础。它们还为开发具有分离代谢物的标准化植物提取物提供了一种方法。
