Gaffron Florian, Tilch Andrea, Grüttner Cordula, Kowalski Anja, Kramer Martin, Teichgräber Ulf, Hilger And Ingrid
Institute for Diagnostic and Interventional Radiology, Jena University Hospital-Friedrich Schiller University Jena, D-07740 Jena, Germany.
Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
Nanomaterials (Basel). 2020 Mar 24;10(3):596. doi: 10.3390/nano10030596.
Fluorochrome-labelled iron oxide magnetic nanoparticles (MNP) have been of great help in elucidating biological processes. Here, we used dually-fluorochrome-labelled MNP and studied to what extent fluorescence detection could reflect their fate in living animals. One day after application in mice (200 µmol Fe/kg body weight), the fluorescence of the dye attached to the core (DY-730) was very prominent and in agreement with the increase of iron in the liver and spleen of mice, but inconspicuous at time points thereafter. We attribute this fluorescence behavior to early degradation processes of the MNP´s core in the cellular lysosomal compartment. In contrast, the fluorescence of the dye DY-555 stuck to the PEG coating was not detectable in vivo. In summary, labelling of MNP with dyes at their metallic core could be of help when detecting first incidences of MNP biodegradation in vivo, as opposed to dyes attached to the MNP coating.
荧光染料标记的氧化铁磁性纳米颗粒（MNP）在阐明生物过程方面有很大帮助。在此，我们使用了双荧光染料标记的MNP，并研究了荧光检测在多大程度上能够反映它们在活体动物体内的命运。在小鼠体内应用（200 μmol Fe/千克体重）一天后，附着在核心上的染料（DY - 730）的荧光非常显著，并且与小鼠肝脏和脾脏中铁含量的增加一致，但在之后的时间点则不明显。我们将这种荧光行为归因于MNP核心在细胞溶酶体区室中的早期降解过程。相比之下，附着在聚乙二醇（PEG）涂层上的染料DY - 555的荧光在体内无法检测到。总之，与附着在MNP涂层上的染料相比，在MNP的金属核心处用染料进行标记在检测体内MNP生物降解的最初情况时可能会有帮助。
