Laboratoire Matières et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
Laboratoire Matériaux et Phénomènes Quantiques, UMR 7162 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
Small. 2017 Jan;13(2). doi: 10.1002/smll.201602030. Epub 2016 Nov 7.
Proteins implicated in iron homeostasis are assumed to be also involved in the cellular processing of iron oxide nanoparticles. In this work, the role of an endogenous iron storage protein-namely the ferritin-is examined in the remediation and biodegradation of magnetic iron oxide nanoparticles. Previous in vivo studies suggest the intracellular transfer of the iron ions released during the degradation of nanoparticles to endogenous protein cages within lysosomal compartments. Here, the capacity of ferritin cages to accommodate and store the degradation products of nanoparticles is investigated in vitro in the physiological acidic environment of the lysosomes. Moreover, it is questioned whether ferritin proteins can play an active role in the degradation of the nanoparticles. The magnetic, colloidal, and structural follow-up of iron oxide nanoparticles and proteins in lysosome-like medium confirms the efficient remediation of potentially harmful iron ions generated by nanoparticles within ferritins. The presence of ferritins, however, delays the degradation of particles due to a complex colloidal behavior of the mixture in acidic medium. This study exemplifies the important implications of intracellular proteins in processes of degradation and metabolization of iron oxide nanoparticles.
铁稳态相关的蛋白质被认为也参与了氧化铁纳米颗粒的细胞内处理。在这项工作中，研究了一种内源性铁储存蛋白——铁蛋白——在磁性氧化铁纳米颗粒的修复和生物降解中的作用。先前的体内研究表明，在纳米颗粒降解过程中释放的铁离子会在溶酶体腔内转移到内源性蛋白笼中。在这里，在溶酶体的生理酸性环境中，体外研究了铁蛋白笼容纳和储存纳米颗粒降解产物的能力。此外，还质疑铁蛋白是否可以在纳米颗粒的降解中发挥积极作用。铁氧化物纳米颗粒和蛋白质在类溶酶体介质中的磁性、胶体和结构跟踪证实了铁蛋白内有效修复了纳米颗粒产生的潜在有害铁离子。然而，由于混合物在酸性介质中的复杂胶体行为，铁蛋白的存在会延迟颗粒的降解。这项研究说明了细胞内蛋白质在氧化铁纳米颗粒的降解和代谢过程中的重要意义。
