Shchit I Yu, Ignatov K B, Kudryavtseva T Yu, Shishkova N A, Mironova R I, Marinin L I, Mokrievich A N, Kramarov V M, Biketov S F, Dyatlov I A
1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia.
2Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117971 Russia.
Mol Gen Microbiol Virol. 2017;32(2):100-108. doi: 10.3103/S0891416817020094. Epub 2017 Sep 20.
The results of detection and identification of strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasmid DNA targets specific for strains has been demonstrated. No cross reactions with DNA from bacterial strains of other species of the group were detected. The development of tests for anthrax-pathogen detection based on the optimized reaction of loop isothermal DNA amplification is planned. These tests will be convenient for clinical studies and field diagnostics due to the absence of requirements for sophisticated equipment.
本文展示了在优化条件下使用原始引物和耐热DNA聚合酶进行环介导等温DNA扩增（LAMP）反应时菌株的检测和鉴定结果。已证明基于LAMP可对特定菌株的染色体和质粒DNA靶点进行可重复检测。未检测到与该组其他物种细菌菌株的DNA发生交叉反应。计划基于优化的环等温DNA扩增反应开发炭疽病原体检测试验。由于对精密设备无要求，这些试验将便于临床研究和现场诊断。
