Yang Shengjie, Liu Mingchuan, Liang Na, Zhao Qi, Zhang Yuping, Xue Wei, Yang Song
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, P,R, China.
Chem Cent J. 2013 Feb 4;7(1):24. doi: 10.1186/1752-153X-7-24.
Cyrtomium fortumei (J.) Smith is an important Chinese herbal medicine because of its biological functions. However, systematic and comprehensive studies on the phytochemicals from Cyrtomium fortumei (J.) Smith and their bioactivity are limited.
Using the bioassay-guided technique, the ethyl acetate and n-BuOH extracts of the rhizomes of Cyrtomium fortumei (J.) Smith were shown to exhibit good antitumor activities, consequently leading to the isolation of 23 compounds. All compounds were isolated from the plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells MGC-803, PC3, and A375 in vitro by MTT (thiazolyl blue tetrazolium bromide) assay, and the results showed that pimpinellin (3) had potent cytotoxic activities against the three cell lines, with the IC50 values of 14.4 ± 0.3 μM, 20.4 ± 0.5 μM, and 29.2 ± 0.6 μM, respectively. The mechanism of the antitumor action indicated that pimpinellin inhibited the growth of MGC-803 cells via the induction of tumor cell apoptosis, with apoptosis ratio of 27.44% after 72 h of treatment at 20 μM.
This study suggests that most of the compounds from the roots of Cyrtomium fortumei (J.) Smith could inhibit the growth of human carcinoma cells. Moreover, pimpinellin inhibited the growth of tumor cells via the induction of tumor cell apoptosis.
贯众因其生物学功能而成为一种重要的中药材。然而，对贯众植物化学成分及其生物活性的系统全面研究较为有限。
采用生物活性导向技术，结果表明贯众根茎的乙酸乙酯提取物和正丁醇提取物具有良好的抗肿瘤活性，从而分离得到23种化合物。所有化合物均首次从该植物中分离得到。通过MTT（噻唑蓝四氮唑溴盐）法体外研究了这些化合物对肿瘤细胞MGC-803、PC3和A375的抑制活性，结果显示茴芹内酯（3）对这三种细胞系具有较强的细胞毒活性，IC50值分别为14.4±0.3 μM、20.4±0.5 μM和29.2±0.6 μM。抗肿瘤作用机制表明，茴芹内酯通过诱导肿瘤细胞凋亡抑制MGC-803细胞生长，在20 μM处理72 h后凋亡率为27.44%。
本研究表明，贯众根中的大多数化合物可抑制人癌细胞生长。此外，茴芹内酯通过诱导肿瘤细胞凋亡抑制肿瘤细胞生长。
