Youssef Mohamed A, El-Ashker Maged R, Ouda Mohamed F
1Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516 Egypt.
Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt.
Comp Clin Path. 2017;26(5):1123-1128. doi: 10.1007/s00580-017-2496-1. Epub 2017 May 31.
So far, there has been scarce information about the status of immunoglobulins (Ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. The purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. For this reason, 50 native breed water buffaloes were studied. Forty of these buffaloes showed various symptoms of digestive disorders and were allocated into 4 groups of equal sizes (group 1: uncategorized stomatitis; group 2: acute traumatic reticuloperitonitis [TRR]; group 3: acute rumen impaction; and group 4: undifferentiated enteritis). Ten apparently healthy buffaloes were randomly selected and considered as a control group. RNA was firstly extracted from the whole blood then a reverse transcription kits was used to convert the RNA to cDNA. Real-time PCR was used to measure the expression of mRNAs of interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor (TNF)-α, IgG, and IgA, while glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as an internal reference. The results of real-time PCR revealed a significant ( ≤ 0.05) upregulation of the gene expression of IL-1β, IL-6, IL-10, and TNF-α in blood of diseased buffaloes compared with those of controls Animals showing acute TRP had peak values of both IL-6 and IL-10; while those exhibiting enteritis and rumen impaction had the highest values of IL-1β and TNF-α, respectively. The results of qPCR also revealed a significant ( ≤ 0.05) downregulation of both IgG and IgA gene expression in blood of all diseased buffaloes compared with controls The lowest values of both genes were recorded in buffaloes showing acute TRP. The results herein suggest that the tested genes could have a pivotal role in the pathophysiologic mechanism of the underlying diseases.
到目前为止，关于出现消化问题的水牛体内免疫球蛋白（Ig）的状态以及炎性细胞因子的基因表达情况，相关信息还很匮乏。本研究的目的是探究患有各种消化系统疾病的水牛体内一些免疫炎症标志物的基因表达调控情况。因此，对50头本地品种的水牛进行了研究。其中40头水牛表现出各种消化紊乱症状，被分为4组，每组数量相等（第1组：未分类的口腔炎；第2组：急性创伤性网胃炎[TRR]；第3组：急性瘤胃积食；第4组：未分化肠炎）。随机挑选10头看似健康的水牛作为对照组。首先从全血中提取RNA，然后使用逆转录试剂盒将RNA转化为cDNA。采用实时定量PCR检测白细胞介素（IL）-1β、IL-6、IL-10、肿瘤坏死因子（TNF）-α、IgG和IgA的mRNA表达，同时以甘油醛-3-磷酸脱氢酶（GAPDH）作为内参。实时定量PCR结果显示，与对照组相比，患病水牛血液中IL-1β、IL-6、IL-10和TNF-α的基因表达显著上调（≤0.05）。患有急性TRP的动物，IL-6和IL-10均达到峰值；而患有肠炎和瘤胃积食的动物，IL-1β和TNF-α分别达到最高值。qPCR结果还显示，与对照组相比，所有患病水牛血液中IgG和IgA的基因表达均显著下调（≤0.05）。这两个基因的最低值出现在患有急性TRP的水牛中。本文结果表明，所检测的基因可能在相关疾病的病理生理机制中起关键作用。
