验证和鉴定用于 Fc 融合蛋白生产的中国仓鼠卵巢细胞中的参考基因。
Validation and identification of reference genes in Chinese hamster ovary cells for Fc-fusion protein production.
机构信息
Wuya college of Innovation; College of life science and biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China.
出版信息
Exp Biol Med (Maywood). 2020 Apr;245(8):690-702. doi: 10.1177/1535370220914058. Epub 2020 Mar 26.
Abstract
In order to reveal potential genotype-phenotype relationship, RT-qPCR reactions are frequently applied which require validated and reliable reference genes. With the investigation on long-term passage and fed-batch cultivation of CHO cells producing an Fc-fusion protein, four new reference genes-Akr1a1, Gpx1, Aprt, and Rps16, were identified from 20 candidates with the aid of geNorm, NormFinder, BestKeeper, and ΔCt programs and methods. This article provided more verified options in reference gene selection in related research on CHO cells.
摘要
为了揭示潜在的基因型-表型关系，常应用 RT-qPCR 反应，这需要经过验证和可靠的参考基因。通过对生产 Fc 融合蛋白的 CHO 细胞进行长期传代和分批培养的研究，借助 geNorm、NormFinder、BestKeeper 和 ΔCt 程序和方法，从 20 个候选基因中鉴定出了 4 个新的参考基因：Akr1a1、Gpx1、Aprt 和 Rps16。本文为 CHO 细胞相关研究中参考基因的选择提供了更多经过验证的选择。
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