Tang Zhiming, Ding Jicun, Zhai Xiaoxiang, Jing Mengqing, Guan Zhiqiang, Li Yongcong
The First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Department of Dermatology, Xuzhou Hospital affiliated with Nanjing University of Traditional Chinese Medicine, Xuzhou, China.
J Int Med Res. 2020 Mar;48(3):300060520909602. doi: 10.1177/0300060520909602.
Galla chinensis ointment can inhibit the proliferation of keloid fibroblasts and decrease keloid formation. We investigated whether Galla chinensis ointment inhibits keloid fibroblast proliferation through expression of microRNA-21, phosphorylated (p)-phosphatidylinositol 3-kinase (p-PI3K), chromosome 10 neutropenic protein phosphatase (PTEN), protein kinase B (p-Akt), and mammalian target of rapamycin (p-mTOR).
A keloid mouse model and human keloid-derived fibroblasts were developed and treated with Galla chinensis. Immunohistochemistry, western blot, and reverse transcription-PCR were used to detect miR-21, PI3K, PTEN, Akt, and mTOR in keloid tissues.
p-Akt and p-mTOR were highly expressed in the control group, PTEN was highly expressed in the treatment group, and p-PI3K was highly expressed in keloid tissue in both groups. Galla chinensis reduced miR-21 expression and increased mRNA expression in keloid fibroblasts compared with the control group, resulting in increased PTEN protein and decreased p-Akt and p-mTOR protein. Galla chinensis had no effect on p-PI3K.
Galla chinensis might inhibit proliferation of keloid fibroblasts by upregulating PTEN, thus inhibiting expression of miR-21 and downregulating p-Akt and p-mTOR expression. These results confirm the effect of Galla chinensis ointment on fibroblasts and suggest that it could be used to manage keloids clinically.
五倍子软膏可抑制瘢痕疙瘩成纤维细胞的增殖并减少瘢痕疙瘩的形成。我们研究了五倍子软膏是否通过微小RNA-21、磷酸化（p）-磷脂酰肌醇3激酶（p-PI3K）、10号染色体中性粒细胞蛋白磷酸酶（PTEN）、蛋白激酶B（p-Akt）和雷帕霉素哺乳动物靶点（p-mTOR）的表达来抑制瘢痕疙瘩成纤维细胞的增殖。
建立瘢痕疙瘩小鼠模型和人瘢痕疙瘩来源的成纤维细胞，并给予五倍子处理。采用免疫组织化学、蛋白质印迹法和逆转录聚合酶链反应检测瘢痕疙瘩组织中的miR-21、PI3K、PTEN、Akt和mTOR。
p-Akt和p-mTOR在对照组中高表达，PTEN在治疗组中高表达，p-PI3K在两组的瘢痕疙瘩组织中均高表达。与对照组相比，五倍子降低了瘢痕疙瘩成纤维细胞中miR-21的表达并增加了mRNA表达，导致PTEN蛋白增加，p-Akt和p-mTOR蛋白减少。五倍子对p-PI3K无影响。
五倍子可能通过上调PTEN来抑制瘢痕疙瘩成纤维细胞的增殖，从而抑制miR-21的表达并下调p-Akt和p-mTOR的表达。这些结果证实了五倍子软膏对成纤维细胞的作用，并表明其可用于临床治疗瘢痕疙瘩。
