Zhai Xiao-Xiang, Ding Ji-Cun, Tang Zhi-Ming, Li Jing-Guo, Chen Xiang-Hui, Zhang Cui-Xia
Department of Dermatology, Chinese Medicine Hospital of Xuzhou, Xuzhou, Jiangsu 221003, P.R. China.
Department of Plastic Surgery, The Affiliated Xuzhou Center Hospital of Nanjing University of Chinese Medicine, Xuzhou, Jiangsu 221009, P.R. China.
Exp Ther Med. 2017 Feb;13(2):503-506. doi: 10.3892/etm.2016.4017. Epub 2016 Dec 30.
We evaluated the effect of Wubeizi (WBZ) ointment on keloids. Keloid-derived fibroblast primary cultures were used to evaluate the effect of the different concentration of WBZ ointment on the expression of type I and III procollagen in keloid fibroblast primary cultures using dot blot assay. Type I and II precollagen cDNA probes labeled with non-radioactive digoxin were used for dot blot. Cell cultures were divided into 4 groups: The large dose group received 1 g/ml of WBZ, middle dose, and small dose groups received 0.5 and 0.25 g/ml of WBZ, respectively. The control group received serum-free medium without WBZ. Our results showed that type I and III procollagen mRNA expression was reduced significantly in the large dose and middle dose groups compared to the control group. Type I and III procollagen mRNA expression level in the small dose group had no statistically significant difference with the control group. However, the difference between the large dose group and the small dose group was statistically significant. We concluded that WBZ ointment aqueous solution restricted keloid fibroblast proliferation by downregulating the expression of type I and III procollagen and therefore reducing collagen deposition in keloid tissue.
我们评估了蜈黛膏对瘢痕疙瘩的影响。采用瘢痕疙瘩来源的成纤维细胞原代培养，运用斑点印迹法评估不同浓度蜈黛膏对瘢痕疙瘩成纤维细胞原代培养中Ⅰ型和Ⅲ型前胶原表达的影响。使用非放射性地高辛标记的Ⅰ型和Ⅱ型前胶原cDNA探针进行斑点印迹。细胞培养分为4组：大剂量组接受1 g/ml蜈黛膏，中剂量组和小剂量组分别接受0.5 g/ml和0.25 g/ml蜈黛膏。对照组接受不含蜈黛膏的无血清培养基。我们的结果显示，与对照组相比，大剂量组和中剂量组中Ⅰ型和Ⅲ型前胶原mRNA表达显著降低。小剂量组中Ⅰ型和Ⅲ型前胶原mRNA表达水平与对照组无统计学显著差异。然而，大剂量组与小剂量组之间的差异具有统计学意义。我们得出结论，蜈黛膏水溶液通过下调Ⅰ型和Ⅲ型前胶原的表达，从而减少瘢痕疙瘩组织中的胶原沉积，限制瘢痕疙瘩成纤维细胞增殖。
