Muni Swamy Ganjayi, Ramesh Gunturu, Devi Prasad Rendedula, Meriga Balaji
Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
Department of Medicinal Chemistry, GVK Biosciences Pvt. Ltd, IDA Mallapur, Hyderabad, Telangana, India.
Arch Physiol Biochem. 2022 Aug;128(4):938-944. doi: 10.1080/13813455.2020.1740742. Epub 2020 Mar 27.
Inhibition of adipogenesis is crucial and is a key area of research to develop antiobesity drugs. In this study, 3-O-glucoside of kaempferol (astragalin) was isolated from leaves and evaluated for its lipolytic and antiadipogenic activity in 3T3-L1 adipocytes. Astragalin has substantially reduced the triglycerides content and lipid accumulation in 3T3-L1 adipocytes and enhanced the glycerol release in a dose dependent manner. The assay for secreted adipocytokines confirmed that, astragalin at a concentration of 20 µg/mL significantly ( < .01) increased the secretion of adiponectin, but decreased leptin secretion in 3T3-L1 adipocytes. In molecular studies, both the mRNA expression and corresponding protein expression of PPAR-γ, C/EBP-α, FAS, and leptin genes were downregulated while that of adiponectin was upregulated in astragalin treated groups. Taken together, astragalin of promotes lipolysis, suppresses adipogenesis in 3T3-L1 adipocytes, and may be considered as an effective candidate to treat obesity aliments.
抑制脂肪生成至关重要，是开发抗肥胖药物的关键研究领域。在本研究中，从叶片中分离出山柰酚3-O-葡萄糖苷（紫云英苷），并在3T3-L1脂肪细胞中评估其脂解和抗脂肪生成活性。紫云英苷以剂量依赖性方式显著降低了3T3-L1脂肪细胞中的甘油三酯含量和脂质积累，并增强了甘油释放。分泌型脂肪细胞因子检测证实，20µg/mL浓度的紫云英苷显著（<0.01）增加了3T3-L1脂肪细胞中脂联素的分泌，但降低了瘦素分泌。在分子研究中，紫云英苷处理组中PPAR-γ、C/EBP-α、FAS和瘦素基因的mRNA表达及相应蛋白表达均下调，而脂联素的表达上调。综上所述，紫云英苷可促进3T3-L1脂肪细胞的脂解，抑制脂肪生成，可被视为治疗肥胖症的有效候选药物。
