Muniandi Sures Kumar, Ariff Farah Fazwa Md, Pisar Mazura Md, Harun Samsuri Toh, Abdullah Mohd Zaki, Abdullah Fauziah, Hashim Siti Nur Aisyah Mohd, Bahari Syafiqah Nabilah Samsul, Saffie Norhayati
Forestry Biotechnology Division, Forest Research Institute Malaysia, Kepong 52109, Selangor, Malaysia.
Natural Product Division, Forest Research Institute Malaysia, Kepong 52109, Selangor, Malaysia.
Biology (Basel). 2024 Sep 30;13(10):785. doi: 10.3390/biology13100785.
L. is a valuable multipurpose tree species widely planted for centuries due to its high medicinal value and antifungal, antiviral, antidepressant, and anti-inflammatory properties in the food industry. However, its cultivation is hindered by production constraints such as the unavailability of planting material and the inadequate number of high-yielding clones. Thus, a study was initiated to select high-yielding clones in terms of growth and chemical content for the mass propagation of superior moringa trees. Screening on high-yielding clones with high astragalin content was conducted through the high-performance liquid chromatography (HPLC) analysis of moringa leaf extract. Selected genotypes were evaluated for their anti-inflammatory potential through in vitro bioactivity assays of leaf methanol extract. The effects of the rooting hormone, rooting substrates, and size of the cutting on the rooting response of branch cuttings of moringa were investigated. Results found that samples collected from different ecological zones of Peninsular Malaysia show significant variation in terms of astragalin content. The extracts were observed to show considerable variation in biological activity against the pro-inflammatory enzymes. The size of the cuttings had significant effects on the rooting of the cuttings as longer cuttings with bigger diameters rooted better than shorter cuttings with smaller diameters. Several genotypes of with superior phenotypic characteristics and bioactive compounds have been identified. Factors affecting the rooting efficiency and optimal conditions of rooting are suggested, which provides valuable information for the propagation of the superior planting material of moringa. This effort will ensure the sustainable production and supply of good quality raw materials for the production of quality end-products for the food and pharmaceutical industry.
辣木是一种有价值的多用途树种，由于其具有很高的药用价值以及在食品工业中的抗真菌、抗病毒、抗抑郁和抗炎特性，几个世纪以来一直被广泛种植。然而，其种植受到一些生产限制因素的阻碍，例如种植材料难以获取以及高产克隆数量不足。因此，开展了一项研究，以选择在生长和化学成分方面表现优异的高产克隆，用于优质辣木树的大规模繁殖。通过对辣木叶提取物进行高效液相色谱（HPLC）分析，筛选出黄芪苷含量高的高产克隆。通过叶甲醇提取物的体外生物活性测定，对选定的基因型进行抗炎潜力评估。研究了生根激素、生根基质和插穗大小对辣木枝条插穗生根反应的影响。结果发现，从马来西亚半岛不同生态区采集的样本在黄芪苷含量方面存在显著差异。观察到提取物对促炎酶的生物活性有相当大的差异。插穗大小对插穗生根有显著影响，直径较大的较长插穗比较短且直径较小的插穗生根更好。已鉴定出几种具有优良表型特征和生物活性化合物的基因型。提出了影响生根效率的因素和最佳生根条件，这为优质辣木种植材料的繁殖提供了有价值的信息。这项工作将确保为食品和制药行业生产优质最终产品提供可持续的优质原材料供应。
