Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
Anim Reprod Sci. 2020 Apr;215:106335. doi: 10.1016/j.anireprosci.2020.106335. Epub 2020 Feb 27.
The study was conducted to evaluate effects of intrauterine administration of proteolytic enzymes on endometrial inflammation and reproductive performance in postpartum water buffalo cows with subclinical endometritis (SCE). Cows (n = 38) with SCE (≥ 18 % PMN i.e.; polymorphonuclear cells) on day 21 postpartum (21 dpp), were allocated into treatment (TR; n = 19; intrauterine infusion of trypsin, chymotrypsin and papain in 20 ml normal saline on 21 dpp) and control (PC; n = 19; intrauterine administration 20 ml saline) groups. Cows without SCE (< 18 % PMN) were not treated and served as the negative control (NC; n = 30). Ultrasonography and sampling (endometrial cytology, uterine flushing, blood) were conducted on day 21 (before treatment) and 28 postpartum (28 dpp). The PMN % and uterine horn diameter were less on 28 dpp (compared with 21 dpp) in NC and TR group only. Cows with SCE had greater uterine concentrations of interleukin (IL)-1β, IL-8 and tumor necrosis factor (TNF)-α; but lesser IL-10 than NC group on 21 and 28 dpp. There were greater serum IL-1β and TNF-α concentrations on 28 dpp in cows with SCE than NC group. Uterine concentrations of IL-1β were less, whereas IL-6 was greater following enzymatic treatment. Proteolytic enzyme treatment did not result in improvement in pregnancy rate compared with the PC group; however, days to conception were less in TR compared with the other two groups. In conclusion, results indicated a reduction in endometrial inflammation and days nonpregnant after proteolytic enzyme treatment in buffalo cows with SCE.
本研究旨在评估在患有亚临床子宫内膜炎（SCE）的产后水牛牛中宫内给予蛋白水解酶对内毒素性子宫内膜炎和生殖性能的影响。产后第 21 天（21 dpp）患有 SCE（PMN 即多形核白细胞≥18%）的奶牛（n = 38），被分配到治疗（TR；n = 19；在 21 dpp 时宫内输注 20 ml 生理盐水的胰蛋白酶、糜蛋白酶和木瓜蛋白酶）和对照组（PC；n = 19；宫内给予 20 ml 生理盐水）。没有 SCE（PMN < 18%）的奶牛不予治疗，作为阴性对照（NC；n = 30）。产后第 21 天（治疗前）和第 28 天（28 dpp）进行超声检查和取样（子宫内膜细胞学、子宫冲洗、血液）。仅在 NC 和 TR 组中，28 dpp 时 PMN%和子宫角直径比 21 dpp 时更小。患有 SCE 的奶牛在第 21 和 28 天的子宫中具有更高浓度的白细胞介素（IL）-1β、IL-8 和肿瘤坏死因子（TNF）-α；但比 NC 组更少的 IL-10。SCE 组奶牛的血清中在第 28 天的 IL-1β和 TNF-α浓度高于 NC 组。在酶处理后，IL-1β的子宫浓度降低，而 IL-6 增加。与 PC 组相比，蛋白水解酶治疗并未导致妊娠率提高；但是，TR 组的受孕天数少于其他两组。总之，结果表明，在患有 SCE 的水牛牛中，蛋白水解酶治疗可降低子宫内膜炎的严重程度和治疗后未受孕的天数。
