Regional Centre, Lala Lajpat Rai University of Veterinary and Animal Sciences, Karnal, Haryana, India.
Veterinary Gynecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Res Vet Sci. 2023 Sep;162:104951. doi: 10.1016/j.rvsc.2023.104951. Epub 2023 Jul 9.
Our objective was to determine the effects of intrauterine infusion of proteolytic enzymes in buffaloes with subclinical endometritis (SCE) at estrus on the resolution of endometrial inflammation and reproductive performance. Buffaloes at spontaneous estrus (E1) were screened for SCE by endometrial cytology to identify SCE (≥5% PMN, n = 22) and non-SCE (<5% PMNs, n = 14) animals. All buffaloes underwent uterine ultrasonographic examination, low volume uterine lavage (cytokines and acute phase proteins) and blood sampling (cytokines and acute-phase proteins) at E1. On the same day (E1), SCE buffaloes were randomly selected either for intrauterine infusion of proteolytic enzymes (ENY, n = 11) or saline (PC, n = 11). Buffaloes without SCE were kept as untreated control (NC; n = 14). All buffaloes were re-examined and re-sampled during subsequent estrus (E2), inseminated during the following estrus (E3), and assessed for fertility related outcomes. Proteolytic infusion resulted a reduction in uterine PMN (P < 0.01) in SCE buffaloes. The concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-α in uterus, and TNF-α and IL-10 in serum were higher (P < 0.01) at E1 in buffaloes with SCE (PC and ENY) compared to NC. After treatment, uterine IL-1β and TNF-α (P = 0.02), and serum TNF-α and IL-10 were lower within the animals of ENY group (P < 0.01). Before treatment, buffaloes with SCE had higher concentrations (P < 0.01) of serum and uterine amyloid-A and haptoglobin, which decreased (P < 0.01) after treatment in the ENY group. None of the fertility outcomes differ between the treatment groups. In conclusion, intrauterine infusion of proteolytic enzymes reduced endometrial inflammation; however, did not improve reproductive outcomes.
我们的目的是确定发情期患有亚临床子宫内膜炎（SCE）的水牛子宫内输注蛋白水解酶对子宫内膜炎消退和生殖性能的影响。通过子宫内膜细胞学检查筛选自发发情（E1）的水牛，以确定 SCE（PMN≥5%，n=22）和非 SCE（PMN<5%，n=14）动物。所有水牛均在 E1 时进行子宫超声检查、小容量子宫冲洗（细胞因子和急性期蛋白）和采血（细胞因子和急性期蛋白）。同日（E1），随机选择 SCE 水牛进行子宫内蛋白水解酶输注（ENY，n=11）或生理盐水输注（PC，n=11）。无 SCE 的水牛作为未处理对照（NC；n=14）。所有水牛在下一个发情期（E2）再次检查和采样，在下一个发情期（E3）进行授精，并评估与生育相关的结果。蛋白水解酶输注可减少 SCE 水牛的子宫PMN（P<0.01）。SCE（PC 和 ENY）水牛的子宫中白细胞介素（IL）-1β和肿瘤坏死因子（TNF）-α以及血清中的 TNF-α和 IL-10 浓度在 E1 时更高（P<0.01）与 NC 相比。治疗后，ENY 组动物的子宫 IL-1β 和 TNF-α（P=0.02）以及血清 TNF-α和 IL-10 降低（P<0.01）。治疗前，SCE 水牛的血清和子宫淀粉样蛋白 A 和触珠蛋白浓度更高（P<0.01），ENY 组治疗后降低（P<0.01）。治疗组的任何生育结果均无差异。总之，子宫内输注蛋白水解酶可减轻子宫内膜炎；然而，并未改善生殖性能。
