University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic; Sino-Czech Joint Laboratory of Fish Conservation and Biotechnology: Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.
University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic.
Anim Reprod Sci. 2020 Apr;215:106334. doi: 10.1016/j.anireprosci.2020.106334. Epub 2020 Feb 26.
Sterlet Acipenser ruthenus was used to assess egg and embryo development when incubated at 17 °C in Petri dishes placed in a hatchery tank (300 L recirculating dechlorinated water) with incubation occurring in a static tabletop system in an air-conditioned laboratory, or in a 700 L Q-cell incubator. Eggs in each dish were placed in a plastic box with 300 mL dechlorinated water. Separated eggs from three individual females were fertilized using pooled sperm from four males with there being four replicates. There were no differences (P > 0.05) in mean percentages of neurulation and embryos undergoing cleavage for eggs incubated in the hatchery tank and with use of the static tabletop system. Furthermore, there were no differences (P > 0.05) in percentage of embryos undergoing cleavage, neurulation and hatching for each female when eggs were incubated using the two systems. Results indicate a Petri dish placed in a small plastic box with 300 mL of dechlorinated water was adequate for incubation of sterlet eggs. Results of the study also indicate that with the static system: 1) eggs should be fertilized from each female to retain individual identity; 2) eggs should be dispersed in Petri dishes to avoid clumping; 3) water should be changed at 24 h, but not at 48 h (neurulation) post-fertilization; and 4) embryos that do not optimally develop should be removed the day after neurulation (72 h of post-fertilization period) and water should be exchanged every day subsequent to the 48 h time-point post-fertilization.
欧鳇被用来评估在 17°C 下孵化的卵和胚胎发育情况，孵化方式为将卵放在孵化槽中的培养皿中（300 升循环去氯水），孵化在空调实验室中的静态台式系统或在 700 升 Q-cell 孵化器中进行。每个培养皿中的卵都放在一个装有 300 毫升去氯水的塑料盒中。来自三个个体雌鱼的分离卵使用来自四个雄鱼的混合精子受精，每个雌鱼有四个重复。在孵化槽和静态台式系统中孵化的卵，其神经胚形成和卵裂的平均百分比没有差异（P > 0.05）。此外，使用这两种系统孵化时，每个雌鱼的卵裂、神经胚形成和孵化的胚胎百分比没有差异（P > 0.05）。结果表明，在小塑料盒中放置一个装有 300 毫升去氯水的培养皿足以孵化欧鳇卵。研究结果还表明，在静态系统中：1）应该从每个雌鱼的卵中受精以保留个体身份；2）应该将卵分散在培养皿中以避免结块；3）受精后 24 小时应换水，但不应在受精后 48 小时（神经胚形成）换水；4）神经胚形成后第二天应去除发育不理想的胚胎，此后应每天换水，直到受精后 48 小时。
