Somova L, Dashev G, Kamenov V, Kirilov G, Kirjakov A, Doncheva M, Vassileva M
Institute of Endocrinology and Gerontology, Bulgarian Medical Academy, Sofia.
Methods Find Exp Clin Pharmacol. 1988 Nov;10(11):677-82.
Streptozotocin diabetes in rats was complicated by spontaneous hypertension (SHR) and myocardial infarction (MIC), considered as "risk groups". Renal function was assessed on the basis of blood urea nitrogen (BUN) and albuminuria. BUN increased by 36% in Wistar diabetic group, by 100% in SHR + diabetes, and by 51% in MIR + diabetes. Morphologic changes were assessed by estimation of PAS-positive glycosaminoglycans and measurement of vascular wall thickness of glomerular arterioles. The risk groups showed exaggerated tendency for development of diabetic angiopathy. A significant imbalance between TXA2 and prostacyclin was found, which was reflected by TXB2/6-keto-PGF1 alpha (the stable metabolites of TXA2 and prostacyclin, respectively) ratio, which increased by 38% in Wistar diabetic rats, by 61% in SHR + diabetes, and by 133% in MIR + diabetes. These changes correlated very well with increased platelet aggregability (r = 0.70; p less than 0.05) and with increased lipid peroxide level (r = 0.60; p less than 0.05), but neither with total plasma cholesterol (r = 0.20), nor with plasma triglycerides (r = 0.34). Lipid peroxides increased 5-fold in Wistar diabetic rats, 6-fold in SHR + diabetes, and 5.5-fold in MIR + diabetes. A causative relationship between TXA2/PGI2 imbalance and lipid peroxide changes on one hand, and diabetic angiopathy, on the other, was suggested.
大鼠链脲佐菌素糖尿病合并自发性高血压（SHR）和心肌梗死（MIC），被视为“风险组”。基于血尿素氮（BUN）和蛋白尿评估肾功能。Wistar糖尿病组BUN升高36%，SHR + 糖尿病组升高100%，MIR + 糖尿病组升高51%。通过估计PAS阳性糖胺聚糖和测量肾小球小动脉血管壁厚度评估形态学变化。风险组显示出糖尿病性血管病发展的过度倾向。发现TXA2和前列环素之间存在显著失衡，这通过TXB2/6 - 酮 - PGF1α（分别为TXA2和前列环素的稳定代谢产物）比值反映出来，该比值在Wistar糖尿病大鼠中升高38%，在SHR + 糖尿病组中升高61%，在MIR + 糖尿病组中升高133%。这些变化与血小板聚集性增加（r = 0.70；p < 0.05）和脂质过氧化物水平升高（r = 0.60；p < 0.05）密切相关，但与总血浆胆固醇（r = 0.20）和血浆甘油三酯（r = 0.34）均无关。脂质过氧化物在Wistar糖尿病大鼠中增加5倍，在SHR + 糖尿病组中增加6倍，在MIR + 糖尿病组中增加5.5倍。提示一方面TXA2/PGI2失衡与脂质过氧化物变化之间，另一方面与糖尿病性血管病之间存在因果关系。
