Fu Jiafang, Zhong Chuanqing, Zhang Peipei, Zong Gongli, Liu Meng, Cao Guangxiang
Department of Epidemiology, The First Affiliated Hospital of Shandong First Medical University, Jinan, China.
Shandong Medicinal Biotechnology Center, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan, China.
Front Microbiol. 2020 Apr 7;11:627. doi: 10.3389/fmicb.2020.00627. eCollection 2020.
Aquatic environments act as reservoirs of antimicrobial-resistant bacteria and antimicrobial resistance (AMR) genes, and the dissemination of antibiotic resistance from these environments is of increasing concern. In this study, a multidrug-resistant bacterial strain, identified as sp. D18, was isolated from the sea water of an industrial maricultural system in the Yellow Sea, China. Whole-genome sequencing of D18 revealed the presence of a novel 25.8 kb antibiotic resistance island, designated GEI-D18A, which carries several antibiotic resistance genes (ARGs), including , , , (B), , , and three genes. Besides, integrase, transposase, resolvase, and recombinase encoding genes were also identified in GEI-D18A. The transferability of GEI-D18A was confirmed by mating experiments between sp. D18 and 25DN, and efflux pump inhibitor assays also suggested that (B) in GEI-D18A was responsible for tetracycline resistance in both D18 and the transconjugant. This study represents the first characterization of a mobilizable antibiotic resistance island in a species of and provides evidence that spp. could be important reservoirs and vehicles for ARGs in the Yellow Sea area.
水生环境是耐抗菌药物细菌和抗菌药物耐药性（AMR）基因的储存库，这些环境中抗生素耐药性的传播日益受到关注。在本研究中，从中国黄海一个工业化海水养殖系统的海水中分离出一株多重耐药细菌菌株，鉴定为 sp. D18。D18的全基因组测序揭示了一个新的25.8 kb抗生素耐药岛的存在，命名为GEI-D18A，它携带多个抗生素耐药基因（ARGs），包括 、 、 、（B）、 、 ，以及三个 基因。此外，在GEI-D18A中还鉴定出了整合酶、转座酶、解离酶和重组酶编码基因。通过 sp. D18与 25DN之间的接合实验证实了GEI-D18A的可转移性，外排泵抑制剂试验也表明，GEI-D18A中的（B）对D18和接合子中的四环素耐药性均有作用。本研究首次对 属物种中一个可移动的抗生素耐药岛进行了表征，并提供了证据表明 属物种可能是黄海地区ARGs的重要储存库和传播载体。
