• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

32S前体核糖体RNA加工：与U3RNA相互作用及间隔区结构重排的动态模型

32S pre-rRNA processing: a dynamic model for interaction with U3RNA and structural rearrangements of spacer regions.

作者信息

Kupriyanova N S

机构信息

Institute of Molecular Biology, USSR Academy of Sciences, Moscow.

出版信息

Mol Biol Rep. 1988;13(2):91-6. doi: 10.1007/BF00539056.

DOI:10.1007/BF00539056
PMID:3221846
Abstract

A model of rearrangement of 32S pre-rRNA during processing was proposed. The first step of these events is the cotranscriptional interaction of the 3'-half of 5.8S rRNA and adjacent part of the internal transcribed spacer (ITS-2) with the 3'-part of the small nucleolar U3RNA from its 155th to 215th nucleotides (numbered for a rat U3RNA). This interaction prevents formation of intramolecular double-stranded structure between 5'-and 3'-end sequences of 5.8S rRNA. The second step is the appearance of extended hairpin structures in the ITS-2, which leads to a compactisation of the entire 32S-pre-rRNA molecule and to the complex formation between 5.8S rRNA and 28S rRNA sequences as the result of U3RNA displacing. After elimination of ITS-2 sequences from 32S pre-rRNA this complex can be included into ribosomes.

摘要

提出了一个32S前体rRNA加工过程中重排的模型。这些事件的第一步是5.8S rRNA的3'端一半和内部转录间隔区（ITS-2）的相邻部分与小核仁U3RNA从其第155至215个核苷酸（以大鼠U3RNA编号）的3'部分进行共转录相互作用。这种相互作用阻止了5.8S rRNA的5'和3'端序列之间形成分子内双链结构。第二步是ITS-2中出现延伸的发夹结构，这导致整个32S前体rRNA分子紧凑化，并由于U3RNA的置换而导致5.8S rRNA和28S rRNA序列之间形成复合物。从32S前体rRNA中去除ITS-2序列后，该复合物可被纳入核糖体。

相似文献

1
32S pre-rRNA processing: a dynamic model for interaction with U3RNA and structural rearrangements of spacer regions.32S前体核糖体RNA加工：与U3RNA相互作用及间隔区结构重排的动态模型
Mol Biol Rep. 1988;13(2):91-6. doi: 10.1007/BF00539056.
2
Processing of mammalian rRNA precursors at the 3' end of 18S rRNA. Identification of cis-acting signals suggests the involvement of U13 small nucleolar RNA.哺乳动物18S rRNA 3'端rRNA前体的加工。顺式作用信号的鉴定表明U13小核仁RNA参与其中。
Eur J Biochem. 1996 Dec 1;242(2):206-13. doi: 10.1111/j.1432-1033.1996.0206r.x.
3
Trypanosoma brucei 5'ETS A'-cleavage is directed by 3'-adjacent sequences, but not two U3 snoRNA-binding elements, which are all required for subsequent pre-small subunit rRNA processing events.布氏锥虫5'ETS A'切割由3'相邻序列引导，但不由两个U3小核仁RNA结合元件引导，而这两个元件对于随后的小亚基前体核糖体RNA加工事件都是必需的。
J Mol Biol. 2001 Nov 2;313(4):733-49. doi: 10.1006/jmbi.2001.5078.
4
snoRNA nuclear import and potential for cotranscriptional function in pre-rRNA processing.小核仁RNA的核输入及其在核糖体RNA前体加工中的共转录功能潜力。
RNA. 2001 Feb;7(2):207-19. doi: 10.1017/s1355838201001625.
5
Processing of truncated mouse or human rRNA transcribed from ribosomal minigenes transfected into mouse cells.对从小鼠核糖体小基因转录而来的截短型小鼠或人核糖体RNA（rRNA）在转染到小鼠细胞后的加工过程。
Mol Cell Biol. 1994 Jun;14(6):4044-56. doi: 10.1128/mcb.14.6.4044-4056.1994.
6
Recognition signals for mouse pre-rRNA processing. A potential role for U3 nucleolar RNA.小鼠前体核糖体RNA加工的识别信号。U3核仁RNA的潜在作用。
Mol Biol Rep. 1983 May;9(1-2):79-86. doi: 10.1007/BF00777477.
7
[Secondary structure of pre-rRNA 41S spacers and its possible role in determining the processing sites].[前体核糖体RNA 41S间隔区的二级结构及其在确定加工位点中的可能作用]
Mol Biol (Mosk). 1989 Nov-Dec;23(6):1611-5.
8
Three small nucleolar RNAs that are involved in ribosomal RNA precursor processing.三种参与核糖体RNA前体加工的小核仁RNA。
Proc Natl Acad Sci U S A. 1997 May 13;94(10):4972-7. doi: 10.1073/pnas.94.10.4972.
9
Dynamic conformational model for the role of ITS2 in pre-rRNA processing in yeast.酵母中ITS2在rRNA前体加工过程中作用的动态构象模型
RNA. 2002 Jun;8(6):786-97. doi: 10.1017/s1355838202023063.
10
Alternative pre-rRNA processing pathways in human cells and their alteration by cycloheximide inhibition of protein synthesis.人类细胞中替代性前体核糖体RNA加工途径及其受环己酰亚胺抑制蛋白质合成的影响
Eur J Biochem. 1993 Feb 15;212(1):211-5. doi: 10.1111/j.1432-1033.1993.tb17652.x.

引用本文的文献

1
Genetic diversity and effect of temperature and pH on the growth of Macrophomina phaseolina isolates from sunflower fields in Hungary.匈牙利向日葵田中茎点霉分离株的遗传多样性及温度和 pH 值对其生长的影响。
Mol Biol Rep. 2012 Mar;39(3):3259-69. doi: 10.1007/s11033-011-1094-6. Epub 2011 Jun 22.
2
U3 small nuclear RNA can be psoralen-cross-linked in vivo to the 5' external transcribed spacer of pre-ribosomal-RNA.U3小核RNA在体内可被补骨脂素交联到核糖体前体RNA的5'外部转录间隔区。
Proc Natl Acad Sci U S A. 1989 Sep;86(17):6523-7. doi: 10.1073/pnas.86.17.6523.
3
Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.

本文引用的文献

1
Chemical modification studies and the secondary structure of HeLa cell 5.8S rRNA.海拉细胞5.8S核糖体RNA的化学修饰研究及其二级结构
Nucleic Acids Res. 1980 Oct 10;8(19):4521-34. doi: 10.1093/nar/8.19.4521.
2
Multiple ribosomal RNA cleavage pathways in mammalian cells.哺乳动物细胞中的多种核糖体RNA切割途径。
Nucleic Acids Res. 1981 Oct 10;9(19):4951-66. doi: 10.1093/nar/9.19.4951.
3
Substitutions, insertions, and deletions in two highly conserved U3 RNA species.两种高度保守的U3 RNA物种中的替换、插入和缺失。
非洲爪蟾原纤维蛋白的分子克隆，原纤维蛋白是一种保守的U3小核核糖核蛋白，可被自身免疫性疾病患者的抗血清识别。
Mol Cell Biol. 1990 Jan;10(1):430-4. doi: 10.1128/mcb.10.1.430-434.1990.
4
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.U3小核仁RNA的缺失会抑制酵母前核糖体RNA 5'外部转录间隔区的切割，并损害18S核糖体RNA的形成。
EMBO J. 1991 Dec;10(13):4231-9. doi: 10.1002/j.1460-2075.1991.tb05001.x.
J Biol Chem. 1980 Jul 25;255(14):7029-33.
4
Structure of mouse rRNA precursors. Complete sequence and potential folding of the spacer regions between 18S and 28S rRNA.小鼠核糖体RNA前体的结构。18S与28S核糖体RNA之间间隔区的完整序列及潜在折叠。
Nucleic Acids Res. 1983 May 25;11(10):3375-91. doi: 10.1093/nar/11.10.3375.
5
Nucleotide sequence of the region between the 18S rRNA sequence and the 28S rRNA sequence of rat ribosomal DNA.大鼠核糖体DNA的18S rRNA序列与28S rRNA序列之间区域的核苷酸序列。
Nucleic Acids Res. 1982 Jun 25;10(12):3667-80. doi: 10.1093/nar/10.12.3667.
6
Nucleotide sequence through the 18S-28S intergene region of a vertebrate ribosomal transcription unit.脊椎动物核糖体转录单位18S - 28S基因间隔区的核苷酸序列。
Nucleic Acids Res. 1980 Dec 20;8(24):5993-6005. doi: 10.1093/nar/8.24.5993.
7
Processing of the large rRNA precursor: two proposed categories of RNA-RNA interactions in eukaryotes.大核糖体RNA前体的加工：真核生物中两类RNA-RNA相互作用的提议
J Mol Evol. 1984;20(3-4):362-7. doi: 10.1007/BF02104742.
8
Multiple states of U3 RNA in Novikoff hepatoma nucleoli.诺维科夫肝癌核仁中U3 RNA的多种状态
Biochemistry. 1984 Nov 6;23(23):5421-5. doi: 10.1021/bi00318a007.
9
A universal model for the secondary structure of 5.8S ribosomal RNA molecules, their contact sites with 28S ribosomal RNAs, and their prokaryotic equivalent.5.8S核糖体RNA分子二级结构、其与28S核糖体RNA的接触位点以及它们的原核生物对应物的通用模型。
Nucleic Acids Res. 1984 Oct 11;12(19):7479-502. doi: 10.1093/nar/12.19.7479.
10
Recognition signals for mouse pre-rRNA processing. A potential role for U3 nucleolar RNA.小鼠前体核糖体RNA加工的识别信号。U3核仁RNA的潜在作用。
Mol Biol Rep. 1983 May;9(1-2):79-86. doi: 10.1007/BF00777477.