海拉细胞5.8S核糖体RNA的化学修饰研究及其二级结构
Chemical modification studies and the secondary structure of HeLa cell 5.8S rRNA.
作者信息
Kelly J M, Maden B E
出版信息
Nucleic Acids Res. 1980 Oct 10;8(19):4521-34. doi: 10.1093/nar/8.19.4521.
Abstract
Various secondary structure models have been proposed for 5.8 S rRNA. In this paper HeLa cell 5.8 S rRNA is shown to possess several sites that are reactive to carbodiimide at 25 degrees, and other regions that are unreactive. Previous work has established the distribution of reactive and unreactive cytidine residues along the primary structure (11). The secondary structure model of Nazar et al. (7) is fully compatible with the chemical reactivity data whereas other models are partly incompatible. We conclude that the model of Nazar et al. provides the best approximation so far available to the conformation of isolated 5.8 S rRNA. Findings on the effect temperature on the chemical reactivity of different parts of the structure are summarized. The findings described in this paper should provide a basis for examining the specific interaction of 5.8 S rRNA with 28 s rRNA.
摘要
针对5.8 S核糖体RNA，人们已经提出了各种二级结构模型。本文显示，HeLa细胞5.8 S核糖体RNA在25摄氏度下有几个对碳二亚胺反应性的位点，以及其他无反应性的区域。先前的研究已经确定了反应性和无反应性胞嘧啶残基沿一级结构的分布（11）。纳扎尔等人（7）的二级结构模型与化学反应性数据完全相符，而其他模型则部分不相符。我们得出结论，纳扎尔等人的模型为分离的5.8 S核糖体RNA的构象提供了目前可用的最佳近似。总结了温度对该结构不同部分化学反应性影响的研究结果。本文所述的研究结果应为研究5.8 S核糖体RNA与28 S核糖体RNA的特异性相互作用提供依据。
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