Austerberry C F, Yao M C
Department of Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Mol Cell Biol. 1988 Sep;8(9):3947-50. doi: 10.1128/mcb.8.9.3947-3950.1988.
Deletions of specific DNA sequences are known to occur in Tetrahymena thermophila as a developmentally regulated process. Deletions of a particular region (region M) were previously shown to be of two alternative sizes, 0.6 or 0.9 kilobases (kb) (C.F. Austerberry, C.D. Allis, and M.-C. Yao, Proc. Natl. Acad. Sci. USA 81: 7383-7387). In this study, the nucleotide sequences for both deletions were determined. These two deletions share the same right junction, but their left junctions are 0.3 kb apart. An 8-base-pair (bp) sequence is present at both junctions of the 0.6-kb deletion, but only 5 bp of this direct repeat are present at the left junction of the 0.9-kb deletion. Further comparison revealed a common 10-bp sequence near each of the two left junctions and a similar sequence in inverted orientation near the right junction. These sequences may play a role in the developmental regulation of the deletion process.
已知在嗜热四膜虫中，特定DNA序列的缺失作为一种发育调控过程会发生。先前已表明特定区域（区域M）的缺失有两种不同大小，即0.6或0.9千碱基（kb）（C.F.奥斯特伯里、C.D.阿利斯和M.-C.姚，《美国国家科学院院刊》81: 7383 - 7387）。在本研究中，确定了这两种缺失的核苷酸序列。这两种缺失具有相同的右连接点，但它们的左连接点相距0.3 kb。在0.6-kb缺失的两个连接点处都存在一个8碱基对（bp）的序列，但在0.9-kb缺失的左连接点处仅存在该直接重复序列的5 bp。进一步比较发现，在两个左连接点附近各有一个共同的10-bp序列，并且在右连接点附近有一个反向的相似序列。这些序列可能在缺失过程的发育调控中起作用。
