Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Boyce Thompson Institute, Ithaca, NY, USA.
Mol Ecol Resour. 2020 Jul;20(4):995-1006. doi: 10.1111/1755-0998.13159. Epub 2020 Apr 20.
The greenhouse whitefly, Trialeurodes vaporariorum Westwood, is an agricultural pest of global importance. Here we report a 787-Mb high-quality draft genome sequence of T. vaporariorum assembled from PacBio long reads and Hi-C chromatin interaction maps, which has scaffold and contig N50 lengths of 70 Mb and 500 kb, respectively, and contains 18,275 protein-coding genes. About 98.8% of the assembled contigs were placed onto the 11 T. vaporariorum chromosomes. Comparative genomic analysis reveals significantly expanded gene families such as aspartyl proteases in T. vaporariorum compared to Bemisia tabaci Mediterranean (MED) and Middle East-Asia Minor 1 (MEAM1). Furthermore, the cytochrome CYP6 subfamily shows significant expansion in T. vaporariorum and several genes in this subfamily display developmental stage-specific expression patterns. The high-quality T. vaporariorum genome provides a valuable resource for research in a broad range of areas such as fundamental molecular ecology, insect-plant/insect-microorganism or virus interactions and pest resistance management.
温室粉虱，烟粉虱（Trialeurodes vaporariorum Westwood），是一种具有全球重要意义的农业害虫。在这里，我们报道了一个 787-Mb 的高质量 T. vaporariorum 基因组草图序列，该序列是通过 PacBio 长读长和 Hi-C 染色质互作图谱组装而成的，其支架和 contig N50 长度分别为 70 Mb 和 500 kb，包含 18275 个蛋白质编码基因。大约 98.8%的组装 contig 被定位到 11 条 T. vaporariorum 染色体上。比较基因组分析表明，与烟粉虱地中海型（MED）和中东-亚洲小种 1 型（MEAM1）相比，T. vaporariorum 中包含显著扩张的基因家族，如天冬氨酸蛋白酶。此外，细胞色素 CYP6 亚家族在 T. vaporariorum 中也有显著扩张，该亚家族中的几个基因表现出发育阶段特异性表达模式。高质量的 T. vaporariorum 基因组为广泛领域的研究提供了宝贵的资源，如基础分子生态学、昆虫-植物/昆虫-微生物或病毒相互作用以及害虫抗性管理。
