Department of Laboratory Diagnostics, Division of Laboratory Diagnostics, Faculty of Pharmacy, Wroclaw, Poland.
Mol Reprod Dev. 2020 May;87(5):515-524. doi: 10.1002/mrd.23340. Epub 2020 Mar 28.
Male infertility is becoming a rapidly growing problem around the world, mainly in the highly developed countries. Seminal proteome composition seems to be one of the crucial factors of the proper course of fertilization - clusterin (CLU) is among the most important ones. CLU, as one of the crucial seminal plasma glycoproteins, plays a very important role in sperm capacitation and immune tolerance in the female reproductive tract. CLU is also known as a sensitive marker of oxidative stress. It has six n-glycosylation sites and also exhibits chaperone activity. An analysis of changes in the profile and degree of CLU glycosylation may shed some new light on the molecular mechanisms of the fertilization process and may be used as an additional diagnostic marker of male fertility. This study constitutes a review of the recently available literature concerning human seminal CLU, including changes in its glycosylation, analyzed in the context of human reproduction.
男性不育症正成为全球范围内一个迅速增长的问题，主要集中在高度发达的国家。精液蛋白组的组成似乎是受精过程的关键因素之一——CLU（簇蛋白）就是其中最重要的因素之一。CLU 作为最重要的精浆糖蛋白之一，在精子获能和女性生殖道免疫耐受中发挥着非常重要的作用。CLU 也被认为是氧化应激的敏感标志物。它有六个 N-糖基化位点，还具有伴侣蛋白活性。分析 CLU 糖基化程度和模式的变化可能会为受精过程的分子机制提供一些新的认识，并可能作为男性生育力的附加诊断标志物。本研究综述了最近关于人精液 CLU 的文献，包括其糖基化变化，并从人类生殖的角度进行了分析。
