• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

证据表明，硬脊膜脂肪组织来源的干细胞的成骨和神经生成分化能力比皮下细胞更为明显。

Evidence that osteogenic and neurogenic differentiation capability of epidural adipose tissue-derived stem cells was more pronounced than in subcutaneous cells.

机构信息

Department of Neurological Sciences, Marmara University, İstanbul, Turkey

Department of Neurosurgery, İstanbul Education Research Hospital, Ministry of Health, İstanbul, Turkey

出版信息

Turk J Med Sci. 2020 Dec 17;50(8):1825-1837. doi: 10.3906/sag-2001-76.

DOI:10.3906/sag-2001-76
PMID:32222128
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7775714/
Abstract

BACKGROUND/AIM: The management of dura-related complications, such as the repairment of dural tears and reconstruction of large dural defects, remain the most challenging subjects of neurosurgery. Numerous surgical techniques and synthetic or autologous adjuvant materials have emerged as an adjunct to primary dural closure, which may result in further complications or side effects. Therefore, the subcutaneous autologous free adipose tissue graft has been recommended for the protection of the central nervous system and repairment of the meninges. In addition, human adipose tissue is also a source of multipotent stem cells. However, epidural adipose tissue seems more promising than subcutaneous because of the close location and intercellular communication with the spinal cord. Herein, it was aimed to define differentiation capability of both subcutaneous and epidural adipose tissue-derived stem cells (ASCs).

MATERIALS AND METHODS

Human subcutaneous and epidural adipose tissue specimens were harvested from the primary incisional site and the lumbar epidural space during lumbar spinal surgery, and ASCs were isolated.

RESULTS

The results indicated that both types of ASCs expressed the cell surface markers, which are commonly expressed stem cells; however, epidural ASCs showed lower expression of CD90 than the subcutaneous ASCs. Moreover, it was demonstrated that the osteogenic and neurogenic differentiation capability of epidural adipose tissue-derived ASCs was more pronounced than that of the subcutaneous ASCs.

CONCLUSION

Consequently, the impact of characterization of epidural ASCs will allow for a new understanding for dural as well as central nervous system healing and recovery after an injury.

摘要

背景/目的：硬脑膜相关并发症的处理，如硬脑膜撕裂的修复和大硬脑膜缺损的重建，仍然是神经外科最具挑战性的课题。许多外科技术和合成或自体辅助材料已作为硬脑膜直接缝合的辅助手段出现，这可能导致进一步的并发症或副作用。因此，建议使用皮下自体游离脂肪组织移植物来保护中枢神经系统和修复脑膜。此外，人体脂肪组织也是多能干细胞的来源。然而，由于与脊髓的位置接近和细胞间通讯，硬膜外脂肪组织似乎比皮下脂肪组织更有前途。在此，旨在定义皮下和硬膜外脂肪组织来源的干细胞（ASCs）的分化能力。

材料和方法

从腰椎手术的原发性切口部位和腰椎硬膜外间隙采集人皮下和硬膜外脂肪组织标本，并分离 ASC。

结果

结果表明，两种类型的 ASC 均表达了常见的干细胞表面标志物；然而，硬膜外 ASC 表达的 CD90 低于皮下 ASC。此外，证明硬膜外脂肪组织衍生的 ASC 的成骨和神经发生分化能力强于皮下 ASC。

结论

因此，对硬膜外 ASC 的特征的研究将为硬脑膜以及中枢神经系统损伤后的愈合和恢复提供新的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/0a994c922f5b/turkjmedsci-50-1825-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/bf60de91159c/turkjmedsci-50-1825-fig001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/e8748d7f9dea/turkjmedsci-50-1825-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/eac46121e2d0/turkjmedsci-50-1825-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/670118d6b3de/turkjmedsci-50-1825-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/ee6736299eb5/turkjmedsci-50-1825-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/83824e3f185e/turkjmedsci-50-1825-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/0a994c922f5b/turkjmedsci-50-1825-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/bf60de91159c/turkjmedsci-50-1825-fig001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/e8748d7f9dea/turkjmedsci-50-1825-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/eac46121e2d0/turkjmedsci-50-1825-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/670118d6b3de/turkjmedsci-50-1825-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/ee6736299eb5/turkjmedsci-50-1825-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/83824e3f185e/turkjmedsci-50-1825-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e1a/7775714/0a994c922f5b/turkjmedsci-50-1825-fig007.jpg

相似文献

1
Evidence that osteogenic and neurogenic differentiation capability of epidural adipose tissue-derived stem cells was more pronounced than in subcutaneous cells.证据表明，硬脊膜脂肪组织来源的干细胞的成骨和神经生成分化能力比皮下细胞更为明显。
Turk J Med Sci. 2020 Dec 17;50(8):1825-1837. doi: 10.3906/sag-2001-76.
2
Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation.电离辐射后脂肪来源干细胞增殖与分化的系列变化
Stem Cell Res Ther. 2016 Aug 17;7(1):117. doi: 10.1186/s13287-016-0378-0.
3
Role of gender and anatomical region on induction of osteogenic differentiation of human adipose-derived stem cells.性别和解剖区域对人脂肪来源干细胞成骨分化诱导的作用
Ann Plast Surg. 2008 Mar;60(3):306-22. doi: 10.1097/SAP.0b013e3180621ff0.
4
Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue.冷冻保存对来自皮下和髌下脂肪组织的犬多能基质细胞的影响。
Stem Cell Rev Rep. 2016 Apr;12(2):257-68. doi: 10.1007/s12015-015-9634-4.
5
Comparison of therapeutic effects of mesenchymal stem cells derived from superficial and deep subcutaneous adipose tissues.比较来源于浅部和深部皮下脂肪组织的间充质干细胞的治疗效果。
Stem Cell Res Ther. 2023 May 4;14(1):121. doi: 10.1186/s13287-023-03350-3.
6
Function of microRNAs in the Osteogenic Differentiation and Therapeutic Application of Adipose-Derived Stem Cells (ASCs).微小 RNA 在脂肪来源干细胞（ASCs）成骨分化中的功能及其治疗应用。
Int J Mol Sci. 2017 Dec 2;18(12):2597. doi: 10.3390/ijms18122597.
7
Gene expression profiles of human subcutaneous and visceral adipose-derived stem cells.人皮下及内脏脂肪来源干细胞的基因表达谱
Cell Biochem Funct. 2016 Dec;34(8):563-571. doi: 10.1002/cbf.3228. Epub 2016 Nov 17.
8
Osteogenic differentiation potential of adipose-derived stem cells from ovariectomized mice.去卵巢小鼠脂肪来源干细胞的成骨分化潜能
Cell Prolif. 2017 Apr;50(2). doi: 10.1111/cpr.12328. Epub 2017 Jan 16.
9
Porcine adipose-derived stem cells from buccal fat pad and subcutaneous adipose tissue for future preclinical studies in oral surgery.用于口腔外科未来临床前研究的颊脂垫和皮下脂肪来源的猪脂肪干细胞。
Stem Cell Res Ther. 2013;4(6):148. doi: 10.1186/scrt359.
10
Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells.心肌缺血患者的胸腺脂肪：多能基质细胞的新来源。
PLoS One. 2015 Dec 10;10(12):e0144401. doi: 10.1371/journal.pone.0144401. eCollection 2015.

引用本文的文献

1
Immortalization of epidural fat-derived mesenchymal stem cells: characterization and adipocyte differentiation potential.硬膜外脂肪来源间充质干细胞的永生化：表征及脂肪细胞分化潜能
World J Stem Cells. 2025 Jan 26;17(1):98777. doi: 10.4252/wjsc.v17.i1.98777.
2
Therapeutic potential of small extracellular vesicles derived from mesenchymal stem cells for spinal cord and nerve injury.间充质干细胞来源的小细胞外囊泡对脊髓和神经损伤的治疗潜力
Front Cell Dev Biol. 2023 Mar 22;11:1151357. doi: 10.3389/fcell.2023.1151357. eCollection 2023.

本文引用的文献

1
Anatomical, Physiological, and Functional Diversity of Adipose Tissue.脂肪组织的解剖、生理和功能多样性。
Cell Metab. 2018 Jan 9;27(1):68-83. doi: 10.1016/j.cmet.2017.12.002.
2
Hippo pathway coactivators Yap and Taz are required to coordinate mammalian liver regeneration.Hippo 通路共激活因子 Yap 和 Taz 对于协调哺乳动物肝脏再生是必需的。
Exp Mol Med. 2018 Jan 5;50(1):e423. doi: 10.1038/emm.2017.205.
3
The Epidural Ligaments (of Hofmann): A Comprehensive Review of the Literature.（霍夫曼）硬膜外韧带：文献综述
Cureus. 2016 Sep 13;8(9):e779. doi: 10.7759/cureus.779.
4
Adipose Tissue-Derived Stem Cells in Regenerative Medicine.再生医学中的脂肪组织来源干细胞
Transfus Med Hemother. 2016 Jul;43(4):268-274. doi: 10.1159/000448180. Epub 2016 Jul 26.
5
A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells.CD90（THY-1）表达的降低导致间充质基质细胞分化增加。
Stem Cell Res Ther. 2016 Jul 28;7(1):97. doi: 10.1186/s13287-016-0359-3.
6
Dermal Adipocytes: From Irrelevance to Metabolic Targets?真皮脂肪细胞：从无关紧要到成为代谢靶点？
Trends Endocrinol Metab. 2016 Jan;27(1):1-10. doi: 10.1016/j.tem.2015.11.002. Epub 2015 Nov 29.
7
Different isolation methods alter the gene expression profiling of adipose derived stem cells.不同的分离方法会改变脂肪来源干细胞的基因表达谱。
Int J Med Sci. 2014 Mar 6;11(4):391-403. doi: 10.7150/ijms.7697. eCollection 2014.
8
Imaging white adipose tissue with confocal microscopy.用共聚焦显微镜对白色脂肪组织进行成像。
Methods Enzymol. 2014;537:17-30. doi: 10.1016/B978-0-12-411619-1.00002-1.
9
Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.脂肪基质细胞包含源自神经嵴的表型不同的脂肪生成祖细胞。
PLoS One. 2013 Dec 31;8(12):e84206. doi: 10.1371/journal.pone.0084206. eCollection 2013.
10
The developmental origins of adipose tissue.脂肪组织的发育起源。
Development. 2013 Oct;140(19):3939-49. doi: 10.1242/dev.080549.