King A, Loke Y W
Department of Pathology, University of Cambridge, Cambridge, UK.
Placenta. 1988 Sep-Oct;9(5):513-21. doi: 10.1016/0143-4004(88)90024-0.
A panel of monoclonal antibodies directed at fucosylated and sialylated carbohydrates on the Type 1 and Type 2 blood group precursor chains was used in an immunohistological study of trophoblast subpopulations. Formalin-fixed paraffin-embedded sections of normal trophoblast throughout pregnancy, hydatidiform moles and gestational choriocarcinoma were examined. Sialyl-Lex was localized in extravillous trophoblast populations in normal and molar pregnancies and choriocarcinoma tumour cells. After neuraminidase treatment, Lex was identified in similar cell populations. Although Lea was identified in syncytial trophoblast cells at the maternofetal interface, no staining was seen for sialyl-Lea. Villous trophoblast did not stain with any of the monoclonal antibodies used. Our results underline the divergent differentiation of trophoblast into villous and invasive extravillous subpopulations. The analogy with tumour cells is discussed.
一组针对1型和2型血型前体链上岩藻糖基化和唾液酸化碳水化合物的单克隆抗体被用于滋养层亚群的免疫组织学研究。对整个孕期正常滋养层、葡萄胎和妊娠绒毛膜癌的福尔马林固定石蜡包埋切片进行了检查。唾液酸化路易斯抗原（Sialyl-Lex）定位于正常和葡萄胎妊娠的绒毛外滋养层细胞群以及绒毛膜癌肿瘤细胞中。经神经氨酸酶处理后，路易斯抗原（Lex）在类似细胞群中被识别。尽管在母胎界面的合体滋养层细胞中鉴定出了Lea，但未观察到唾液酸化Lea的染色。绒毛滋养层未被所用的任何一种单克隆抗体染色。我们的结果强调了滋养层向绒毛和侵袭性绒毛外亚群的不同分化。并讨论了与肿瘤细胞的相似性。
