Department of Molecular Medicine, University of Padova, Via Gabelli, 63, IT-35121 Padova, Italy; Department of Microbiology, The Public Health Agency of Sweden, Nobels Väg 18, SE-171 82 Solna, Sweden.
Department of Microbiology, The Public Health Agency of Sweden, Nobels Väg 18, SE-171 82 Solna, Sweden; Department for Laboratory Medicine, Karolinska University Hospital and KI, SE-14186 Huddinge, Sweden.
Ticks Tick Borne Dis. 2020 Jul;11(4):101417. doi: 10.1016/j.ttbdis.2020.101417. Epub 2020 Mar 19.
Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne viral disease caused by the orthonairovirus CCHF virus (CCHFV). Ticks of the genus Hyalomma are the viral reservoir and they represent the main vector transmitting the virus to their hosts during blood feeding. However, how CCHFV replicates in its natural arthropod host cells and the nature of virus/host interactions are still largely unknown. With the aim of developing tools for use in this field, we identified and validated expression of four candidate endogenous control tick genes in a Hyalomma anatolicum-derived cell line. These genes will be useful for normalization of viral/cellular transcripts in infection/expression studies or as internal controls in molecular epidemiology surveys of pathogens transmitted by Hyalomma ticks.
克里米亚-刚果出血热（CCHF）是一种由正纳罗病毒克里米亚-刚果出血热病毒（CCHFV）引起的新兴蜱传病毒性疾病。璃眼蜱属的蜱是该病毒的储存宿主，它们在吸血时将病毒传播给宿主，是主要的传播媒介。然而，CCHFV 在其自然节肢动物宿主细胞中的复制方式以及病毒/宿主相互作用的性质在很大程度上仍然未知。为了开发该领域的工具，我们在一种源于璃眼蜱属的细胞系中鉴定和验证了四个候选内源性控制蜱基因的表达。这些基因将有助于在感染/表达研究中对病毒/细胞转录物进行归一化，或作为由璃眼蜱传播的病原体分子流行病学调查的内参。
