Research Institute of Experimental Biology and Medicine, Voronezh N. N. Burdenko State Medical University, Voronezh, Russia.
Institute of Hematopathology, Fangdieckstr. 75a, 22547, Hamburg, Germany.
Histochem Cell Biol. 2020 Jul;154(1):21-40. doi: 10.1007/s00418-020-01875-9. Epub 2020 Mar 28.
This article presents 20 combinations of histochemical stainings for the determination of mast cell co-localization with the fibrous component of the connective tissue in the fibrillogenesis course. Best results were obtained using metachromatic detection of mast cells in combination with silver or picro-fuchsin impregnation, staining with brilliant green using van Gieson staining, and a combination of aniline blue staining with neutral red. Proposed variants of histochemical protocols open up new opportunities to analyze the participation of mast cells in extracellular matrix remodeling of the tissue microenvironment in the course of adaptive and pathological processes. Results obtained expand the current theoretical views of the process of fibrillogenesis in the extracellular matrix. They also shed new light on the participation of mast cell secretion components in the molecular mechanisms of fiber formation.
本文提出了 20 种组合的组织化学染色方法，用于确定肥大细胞与纤维组织的纤维成分在纤维生成过程中的共定位。使用甲苯胺蓝染色与中性红染色相结合、用 van Gieson 染色结合亮绿染色、以及用银或苦味酸浸染结合变色反应检测肥大细胞，可获得最佳结果。所提出的组织化学方案的变体为分析肥大细胞在适应性和病理过程中组织微环境细胞外基质重塑中的参与提供了新的机会。所获得的结果扩展了细胞外基质中纤维生成过程的现有理论观点。它们还揭示了肥大细胞分泌成分在纤维形成的分子机制中的参与。
