Li Xueqing, Chen Yuanzheng, Fu Cong, Li Hongwen, Yang Kun, Bi Jianhai, Huo Ran
Department of Plastic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
Department of Burns & Plastic Surgery, Linyi People's Hospital, Linyi, PR China.
Epigenomics. 2020 Jun;12(11):893-905. doi: 10.2217/epi-2020-0060. Epub 2020 Mar 30.
This study was conducted to reveal epigenetic landscape in infantile hemangiomas (IHs) and identify transcription factors (TFs) and their downstream genes active in IHs. We performed Assay for Transposase Accessible Chromatin (ATAC-seq) with RNA-seq in three pairs of IHs and their adjacent normal tissues. Functions of candidate TFs were investigated in human umbilical vein endothelial cells (HUVECs). Chromatin of IH tissues is less compact. Some candidate genes and TFs were identified. In HUVECs, SPDEF inhibited cell viability and tube formation, and promoted apoptosis; SOX4 exerted the opposite effect. SPDEF may act through , and ; SOX4 may act through and . Epigenetics plays a role in IHs. SPDEF and SOX4 may act in IHs.
本研究旨在揭示婴儿血管瘤（IHs）的表观遗传景观，并鉴定在IHs中活跃的转录因子（TFs）及其下游基因。我们在三对IHs及其相邻正常组织中进行了转座酶可及染色质分析（ATAC-seq）和RNA测序。在人脐静脉内皮细胞（HUVECs）中研究了候选TFs的功能。IH组织的染色质较疏松。鉴定出了一些候选基因和TFs。在HUVECs中，SPDEF抑制细胞活力和管形成，并促进细胞凋亡；SOX4则发挥相反的作用。SPDEF可能通过 、 和 起作用；SOX4可能通过 和 起作用。表观遗传学在IHs中起作用。SPDEF和SOX4可能在IHs中发挥作用。
