Biotechnology Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran; Biomedical Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Biotechnology Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran; Biomedical Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Int J Biol Macromol. 2020 Jul 15;155:119-130. doi: 10.1016/j.ijbiomac.2020.03.194. Epub 2020 Mar 26.
Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing. However, electrospinning of GA is a bottleneck due to its physico-chemical properties. The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength. For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition. The synthesis of AgNPs was supported by UV-vis and ICP analyses. The structure of mat and its constituents were characterized by FE-SEM, XRD and FTIR. The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m.day, respectively. The antimicrobial activity of mat against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans was proved. Moreover, the cytotoxicity of mat showed the good biocompatibility for the mouse embryonic fibroblast cells. This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
阿拉伯树胶（GA）是一种具有生物相容性的聚合物，具有作为伤口敷料的必要要求。然而，由于其物理化学性质，GA 的静电纺丝是一个瓶颈。本研究的目的是用 GA 制备具有合适的孔隙率、吸水性、水蒸气透过率和机械强度的抗菌纳米纤维垫。为此，根据可能的最高孔隙率、吸水性和水蒸气透过率，优化了聚己内酯（PCL）涂层 GA-聚乙烯醇（PVA）纳米纤维垫的组成，然后根据该组成制备载银纳米粒子（AgNPs）的纳米纤维垫。AgNPs 的合成得到了 UV-vis 和 ICP 分析的支持。采用 FE-SEM、XRD 和 FTIR 对垫及其成分的结构进行了表征。结果表明，纳米纤维的平均直径在 150 至 250nm 范围内，孔隙率、吸水率和水蒸气透过率分别为 37.34%、547.30%和 2235.50g/m·day。证明了垫对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌的抗菌活性。此外，垫的细胞毒性显示出对小鼠胚胎成纤维细胞的良好生物相容性。本研究介绍了 PCL 涂层 GA-PVA-AgNPs 作为一种有效的抗菌垫，可替代商业伤口敷料。
