Laboratory for Cancer Biology, Department of Medical Oncology and Clinical research, Cancer Institute (WIA), Chennai, Tamilnadu, India.
Department of Medical Oncology and Clinical Research, Cancer Institute (WIA), Chennai, Tamilnadu, India.
Cancer Biomark. 2020;28(3):397-408. doi: 10.3233/CBM-201463.
There has been variability between laboratories in the identification of cancer stem cells (CSCs) markers for epithelial ovarian cancer (EOC). We have evaluated three new surface markers for EOC to identify CSCs precisely.
Three new putative CSCs specific surface markers CD9, CD24 and EPHA1 identified by a bioinformatics approach were evaluated in normal ovary, fallopian tube and ovarian tumours.
The expression of CD9 alone was observed in normal ovarian surface epithelium and fallopian tube whereas CD24 and EPHA1 were not expressed (n= 5). CD24 was expressed in all tumours (N= 101) while CD9 and EPHA1 were expressed in 89 and 71 tumours, respectively. The statistical analysis showed significant correlation of the stage of the disease (p< 0.0001), type of surgery (p< 0.0001) and residual disease (p< 0.0001) with overall survival. Although expression of CD9, CD24 and EPHA1 was observed in the majority of tumours there was no significant correlation with outcome. In patients who underwent primary surgery, increased expression of CD24 significantly correlated with poor survival. The expression of CD24 was significantly reduced (p< 0.002) upon analysis of paired sections from patients prior to surgery and at interval debulking surgery (n= 16).
These findings suggest that overexpression of these new markers may be useful in identifying and targeting ovarian CSCs and CD24 may be a putative CSCs marker in ovarian cancer.
在识别上皮性卵巢癌（EOC）的癌症干细胞（CSC）标志物方面，不同实验室之间存在差异。我们评估了三种新的用于 EOC 的表面标志物，以准确识别 CSC。
通过生物信息学方法鉴定出三种新的上皮性卵巢癌潜在的 CSC 特异性表面标志物 CD9、CD24 和 EPHA1，并对正常卵巢、输卵管和卵巢肿瘤进行评估。
单独表达 CD9 的情况仅在正常卵巢表面上皮和输卵管中观察到，而 CD24 和 EPHA1 未表达（n=5）。CD24 在所有肿瘤（N=101）中表达，而 CD9 和 EPHA1 在 89 和 71 个肿瘤中表达。统计分析显示，疾病分期（p<0.0001）、手术类型（p<0.0001）和残留疾病（p<0.0001）与总生存期有显著相关性。尽管在大多数肿瘤中观察到 CD9、CD24 和 EPHA1 的表达，但与预后无显著相关性。在接受初次手术的患者中，CD24 表达增加与不良预后显著相关。对 16 例患者术前和间隔减瘤手术的配对切片进行分析，发现 CD24 的表达显著降低（p<0.002）。
这些发现表明，这些新标志物的过度表达可能有助于识别和靶向卵巢 CSC，CD24 可能是卵巢癌中的潜在 CSC 标志物。
