Opt Express. 2020 Mar 30;28(7):9738-9752. doi: 10.1364/OE.387027.
In this study, the incorporation between gold modified-tyrosinase (Tyr) enzyme based graphene oxide (GO) thin film with surface plasmon resonance (SPR) technique has been developed for the detection of phenol. SPR signal for the thin film contacted with phenol solution was monitored using SPR technique. From the SPR curve, sensitivity, full width at half maximum (FWHM), detection accuracy (DA) and signal-to-noise ratio (SNR) have been analyzed. The sensor produces a linear response for phenol up to 100 µM with sensitivity of 0.00193° µM. Next, it can be observed that deionized water has the lowest FWHM, with a value of 1.87° and also the highest value of DA. Besides, the SNR of the SPR signal was proportional to the phenol concentrations. Furthermore, the surface morphology of the modified thin film after exposed with phenol solution observed using atomic force microscopy showed a lot of sharp peaks compared to the image before in contact with phenol proved the interaction between the thin film and phenol.
在这项研究中，已开发出一种基于金修饰酪氨酸酶（Tyr）酶的氧化石墨烯（GO）薄膜与表面等离子体共振（SPR）技术相结合的方法，用于检测苯酚。使用 SPR 技术监测与苯酚溶液接触的薄膜的 SPR 信号。从 SPR 曲线中，分析了灵敏度、半峰全宽（FWHM）、检测精度（DA）和信噪比（SNR）。该传感器对苯酚的线性响应高达 100µM，灵敏度为 0.00193°µM。接下来，可以观察到去离子水具有最低的 FWHM，为 1.87°，并且具有最高的 DA 值。此外，SPR 信号的 SNR 与苯酚浓度成正比。此外，使用原子力显微镜观察到暴露于苯酚溶液后的修饰薄膜的表面形态与接触苯酚之前相比显示出许多尖锐的峰，这证明了薄膜与苯酚之间的相互作用。
