School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, Liaoning 114051, China.
School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, Liaoning 114051, China.
Bioelectrochemistry. 2018 Aug;122:174-182. doi: 10.1016/j.bioelechem.2018.04.003. Epub 2018 Apr 9.
The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5×10M to 5×10M, a lower detection limit of 3×10M, a current maximum (I) of 65.8μA and an apparent Michaelis constant (K) of 169.9μM.
研究了基于通过戊二醛（GA）共价键合到氧化石墨烯（GO）修饰的玻碳电极（GCE）上的酪氨酸酶（TYR）的苯酚生物传感器的制造、表征和分析性能。通过原子力显微镜（AFM）和场发射扫描电子显微镜（FE-SEM）研究了修饰电极的表面形态。制备的 TYR/GA/GO/GCE 生物传感器表现出非常好的稳定性、重现性、灵敏度和实际用途。儿茶酚生物传感器的感应线性范围从 5×10^-6 M 到 5×10^-5 M，检测下限为 3×10^-6 M，电流最大值（I）为 65.8μA，表观米氏常数（K）为 169.9μM。
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