基于氧化石墨烯修饰酪氨酸酶电极的高灵敏电化学生物传感器用于检测酚类衍生物。
A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode.
机构信息
School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, Liaoning 114051, China.
School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, Liaoning 114051, China.
出版信息
Bioelectrochemistry. 2018 Aug;122:174-182. doi: 10.1016/j.bioelechem.2018.04.003. Epub 2018 Apr 9.
The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5×10M to 5×10M, a lower detection limit of 3×10M, a current maximum (I) of 65.8μA and an apparent Michaelis constant (K) of 169.9μM.
研究了基于通过戊二醛（GA）共价键合到氧化石墨烯（GO）修饰的玻碳电极（GCE）上的酪氨酸酶（TYR）的苯酚生物传感器的制造、表征和分析性能。通过原子力显微镜（AFM）和场发射扫描电子显微镜（FE-SEM）研究了修饰电极的表面形态。制备的 TYR/GA/GO/GCE 生物传感器表现出非常好的稳定性、重现性、灵敏度和实际用途。儿茶酚生物传感器的感应线性范围从 5×10^-6 M 到 5×10^-5 M，检测下限为 3×10^-6 M，电流最大值（I）为 65.8μA，表观米氏常数（K）为 169.9μM。
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