Department of Physics, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Physics, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Dec 15;263:120202. doi: 10.1016/j.saa.2021.120202. Epub 2021 Jul 21.
Due to the crucial role of dopamine (DA) in health and peripheral nervous systems, it is particularly important to develop an efficient and accurate sensor to monitor and determine DA concentrations for diagnostic purposes and diseases prevention. Up to now, using surface plasmon resonance (SPR) sensors in DA determination is very limited and its application still at the primary stage. In this work, a simple and ultra-sensitive SPR sensor was constructed for DA detection by preparation of chitosan- graphene quantum dots (CS-GQDs) thin film as the sensing layer. Other SPR measurements were conducted using different sensing layers; GQDs, CS for comparison. The proposed thin films were prepared by spin coating technique. The developed CS-GQDs thin film-based SPR sensor was successfully tested in DA concentration range from 0 fM to 1 pM. The designed SPR sensor showed outstanding performance in detecting DA sensitively (S = 0.011°/fM, R = 0.8174) with low detection limit of 1.0 fM has been achieved for the first time. The increased angular shift of SPR dip, narrow full width half maximum of the SPR curves, excellent signal-to-noise ratio and figure of merit, and a binding affinity constant (K) of 2.962 PM demonstrated the potential of this sensor to detect DA with high accuracy. Overall, it was concluded that the proposed sensor would serve as a valuable tool in clinical diagnostic for the serious neurological disorders. This in turns has a significant socio-economic impact.
由于多巴胺（DA）在健康和外周神经系统中的关键作用，因此开发一种用于监测和确定 DA 浓度的高效且准确的传感器对于诊断目的和疾病预防尤为重要。到目前为止，使用表面等离子体共振（SPR）传感器来确定 DA 的应用非常有限，其应用仍处于初级阶段。在这项工作中，通过制备壳聚糖-石墨烯量子点（CS-GQDs）薄膜作为传感层，构建了一种用于 DA 检测的简单且超灵敏的 SPR 传感器。其他 SPR 测量使用不同的传感层进行；GQDs、CS 进行比较。通过旋涂技术制备了所提出的薄膜。所开发的基于 CS-GQDs 薄膜的 SPR 传感器已成功用于检测 0 fM 至 1 pM 范围内的 DA 浓度。设计的 SPR 传感器在检测 DA 方面表现出出色的性能，灵敏度高（S = 0.011°/fM，R = 0.8174），首次实现了低至 1.0 fM 的检测限。SPR 曲线的 SPR 拐点角度位移增加、半峰全宽变窄、信噪比和品质因数优异，以及结合亲和力常数（K）为 2.962 PM，表明该传感器具有高准确度检测 DA 的潜力。总体而言，该传感器可作为临床诊断严重神经疾病的有用工具。这反过来又具有重大的社会经济影响。
