基于羧基化石墨烯氧化物的表面等离子体共振适体传感器用于唐氏综合征筛查的临床应用。

Clinical Application for Screening Down's Syndrome by Using Carboxylated Graphene Oxide-Based Surface Plasmon Resonance Aptasensors.

机构信息

Laboratory of Nano-Photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei City 11677, Taiwan.

Department of Life Science, National Taiwan Normal University, Taipei City 11677, Taiwan.

出版信息

Int J Nanomedicine. 2020 Oct 22;15:8131-8149. doi: 10.2147/IJN.S270938. eCollection 2020.

DOI:10.2147/IJN.S270938

PMID:33144830

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7594198/

Abstract

BACKGROUND

Advanced medical detection technology requires high sensitivity and accuracy to increase the disease detection rate. We showed that carboxyl-functionalized graphene oxide (carboxyl-GO) biosensing materials are capable of accurate detection.

METHODS

We developed a carboxylated GO-based surface plasmon resonance (SPR) aptasensor suitable for screening Down's syndrome in clinical serum. This biosensing material could rapidly and accurately detect hCG protein with a low concentration to identify fetal Down's syndrome. The developed carboxyl-GO-based SPR aptasensor showed excellent sensitivity and limit of detection without the use of antibodies and without any specific preference.

RESULTS

hCG protein detection limits of 1 pM in buffer samples and 1.9 pM in clinical serum samples were achieved. The results showed that the carboxyl-GO-based chip could detect hCG well below the normal physiological level of serum protein (5.0 mIU/mL). High affinity, sensitivity, and better detection limit were obtained in the range of 1.9 pM to 135 pM. The results showed a 5k-fold dilution factor, and that an SPR angle shift of more than 20 millidegrees (m) was associated with a significant risk of fetal Down's syndrome compared to normal pregnant women. The results clearly showed that the detection of hCG protein in serum samples from pregnant women at 12-19 weeks could be used to screen Down's syndrome with high selectivity and sensitivity.

CONCLUSION

Our findings suggest the potential application of carboxyl-GO film in proof-of-concept studies for serum assays as a new type of SPR material. In addition, peptide and carboxyl-GO films may be conducive to the development of future point of care testing and rapid diagnostic devices for other diseases such as cancer.

摘要

背景

先进的医学检测技术需要高灵敏度和准确性，以提高疾病检出率。我们表明，羧基功能化氧化石墨烯（carboxyl-GO）生物传感材料能够进行准确检测。

方法

我们开发了一种基于羧基 GO 的表面等离子体共振（SPR）适体传感器，适用于临床血清中唐氏综合征的筛选。这种生物传感材料能够快速、准确地检测低浓度的 hCG 蛋白，以识别胎儿唐氏综合征。开发的基于羧基 GO 的 SPR 适体传感器具有出色的灵敏度和检测限，无需使用抗体，也没有任何特定偏好。

结果

在缓冲液样品中达到了 1 pM 的 hCG 蛋白检测限，在临床血清样品中达到了 1.9 pM 的检测限。结果表明，基于羧基 GO 的芯片能够在血清蛋白的正常生理水平（5.0 mIU/mL）以下很好地检测 hCG。在 1.9 pM 至 135 pM 的范围内，获得了高亲和力、灵敏度和更好的检测限。结果显示，与正常孕妇相比，hCG 蛋白在血清中的稀释倍数达到 5000 倍，且超过 20 毫弧度（m）的 SPR 角度变化与胎儿唐氏综合征的显著风险相关。结果清楚地表明，在 12-19 周的孕妇血清样本中检测 hCG 蛋白可以用于高选择性和高灵敏度的唐氏综合征筛查。