Lobry Maxime, Loyez Médéric, Hassan Eman M, Chah Karima, DeRosa Maria C, Goormaghtigh Erik, Wattiez Ruddy, Caucheteur Christophe
Opt Express. 2020 Mar 2;28(5):7539-7551. doi: 10.1364/OE.385747.
Tilted fiber Bragg gratings (TFBGs) are now a well-established technology in the scientific literature, bringing numerous advantages, especially for biodetection. Significant sensitivity improvements are achieved by exciting plasmon waves on their metal-coated surface. Nowadays, a large part of advances in this topic relies on new strategies aimed at providing sensitivity enhancements. In this work, TFBGs are produced in both single-mode and multimode telecommunication-grade optical fibers, and their relative performances are evaluated for refractometry and biosensing purposes. TFBGs are biofunctionalized with aptamers oriented against HER2 (Human Epidermal Growth Factor Receptor-2), a relevant protein biomarker for breast cancer diagnosis. In vitro assays confirm that the sensing performances of TFBGs in multimode fiber are higher or identical to those of their counterparts in single-mode fiber, respectively, when bulk refractometry or surface biosensing is considered. These observations are confirmed by numerical simulations. TFBGs in multimode fiber bring valuable practical assets, featuring a reduced spectral bandwidth for improved multiplexing possibilities enabling the detection of several biomarkers.
倾斜光纤布拉格光栅（TFBGs）如今在科学文献中已是一项成熟的技术，具有诸多优势，尤其在生物检测方面。通过在其金属涂层表面激发等离子体波可实现显著的灵敏度提升。如今，该领域的很大一部分进展依赖于旨在提高灵敏度的新策略。在这项工作中，TFBGs在单模和多模电信级光纤中制造，并针对折射测量和生物传感目的评估它们的相对性能。TFBGs用针对HER2（人表皮生长因子受体2）的适体进行生物功能化，HER2是乳腺癌诊断的一种相关蛋白质生物标志物。体外实验证实，当考虑体折射测量或表面生物传感时，多模光纤中TFBGs的传感性能分别高于或等同于单模光纤中TFBGs的传感性能。这些观察结果得到了数值模拟的证实。多模光纤中的TFBGs具有宝贵的实际优势，其光谱带宽减小，改善了复用可能性，能够检测多种生物标志物。
