Lobry Maxime, Loyez Médéric, Chah Karima, Hassan Eman M, Goormaghtigh Erik, DeRosa Maria C, Wattiez Ruddy, Caucheteur Christophe
Electromagnetism and Telecommunication Department, University of Mons, 31 Bld Dolez, 7000 Mons, Belgium.
Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles, Bld du Triomphe 2,1050 Brussels, Belgium.
Biomed Opt Express. 2020 Aug 4;11(9):4862-4871. doi: 10.1364/BOE.401200. eCollection 2020 Sep 1.
In the biomedical detection context, plasmonic tilted fiber Bragg gratings (TFBGs) have been demonstrated to be a very accurate and sensitive sensing tool, especially well-adapted for biochemical detection. In this work, we have developed an aptasensor following a triple strategy to improve the overall sensing performances and robustness. Single polarization fiber (SPF) is used as biosensor substrate while the demodulation is based on tracking a peculiar feature of the lower envelope of the cladding mode resonances spectrum. This method is highly sensitive and yields wavelength shifts several tens of times higher than the ones reported so far based on the tracking of individual modes of the spectrum. An amplification of the response is further performed through a sandwich assay by the use of specific antibodies. These improvements have been achieved on a biosensor developed for the detection of the HER2 () protein, a relevant breast cancer biomarker. These advanced developments can be very interesting for point-of-care biomedical measurements in a convenient practical way.
在生物医学检测领域，表面等离激元倾斜光纤布拉格光栅（TFBGs）已被证明是一种非常精确且灵敏的传感工具，特别适用于生化检测。在这项工作中，我们开发了一种采用三重策略的适配体传感器，以提高整体传感性能和稳健性。单偏振光纤（SPF）用作生物传感器基板，同时基于跟踪包层模共振光谱的下包络的一个特殊特征进行解调。该方法高度灵敏，产生的波长偏移比迄今为止基于光谱单个模式跟踪所报道的偏移高几十倍。通过使用特异性抗体的夹心测定法进一步实现响应放大。这些改进是在为检测HER2（）蛋白（一种相关的乳腺癌生物标志物）而开发的生物传感器上实现的。这些先进的进展对于以方便实用的方式进行即时生物医学测量可能非常有意义。
