Liu Wenya, Cai Tonghui, Li Lingjun, Chen Hui, Chen Ruichao, Zhang Minfen, Zhang Wei, Zhao Li, Xiong Hanzhen, Qin Ping, Gao Xingcheng, Jiang Qingping
Department of Pathology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 510150.
Department of Pathology, the First Affiliated Hospital, Anhui Medical University, Hefei, China 230022.
J Cancer. 2020 Mar 4;11(10):3052-3060. doi: 10.7150/jca.40438. eCollection 2020.
Nasopharyngeal carcinoma (NPC), is one of the most common malignant tumor in southern China and southeast Asia. MYH10 is a coding gene of the NMMHC-IIB protein. Previous studies have shown that MYH10 expression was up-regulated in breast cancer, glioma and meningioma. Moreover, it was targeted by miR200 family. However, no relevant studies have been found in NPC. In present study, we found in 48 NPC specimens, MYH10 level was lower in most cancer areas than that in the adjacent normal tissue. Moreover, the depletion of MYH10 can promote the migration and invasion of NPC. In addition, we demonstrated that miR-200a has the strongest regulation to MYH10 among miR-200 family. miR-200a mimics could decrease MYH10 expression, while miR-200a inhibitor increase MYH10 expression. Next, we found that miR-200a bound directly to MYH10 using Dual-luciferase reporter. Finally, it was demonstrated that siMYH10 could reverse the effect of miR-200a inhibitor on NPC cell migration and invasion. Taken together, it can be concluded that MYH10 is lowly expressed in NPC compared with adjacent tissues, and the loss of MYH10 can promote the migration and invasion of NPC cells; Among the miR-200 family, miR-200a has the strongest regulatory effect on MYH10; MYH10 is a direct target gene of miR200a, and miR200a targets MYH10 to regulate the migration and invasion of NPC cells.
鼻咽癌（NPC）是中国南方和东南亚地区最常见的恶性肿瘤之一。MYH10是NMMHC-IIB蛋白的编码基因。先前的研究表明，MYH10在乳腺癌、神经胶质瘤和脑膜瘤中表达上调。此外，它是miR200家族的靶点。然而，在鼻咽癌中尚未发现相关研究。在本研究中，我们发现，在48例鼻咽癌标本中，大多数癌组织区域的MYH10水平低于相邻正常组织。此外，MYH10的缺失可促进鼻咽癌的迁移和侵袭。此外，我们证明，在miR-200家族中，miR-200a对MYH10的调控作用最强。miR-200a模拟物可降低MYH10的表达，而miR-200a抑制剂则可增加MYH10的表达。接下来，我们使用双荧光素酶报告基因发现miR-200a直接与MYH10结合。最后，证明siMYH10可逆转miR-200a抑制剂对鼻咽癌细胞迁移和侵袭的影响。综上所述，可以得出结论，与相邻组织相比，MYH10在鼻咽癌中低表达，MYH10的缺失可促进鼻咽癌细胞的迁移和侵袭；在miR-200家族中，miR-200a对MYH10的调控作用最强；MYH10是miR200a的直接靶基因，miR200a通过靶向MYH10调控鼻咽癌细胞的迁移和侵袭。
