长链非编码 RNA HCG11 通过吸附 miR-522-3p 抑制非小细胞肺癌的肿瘤发生，上调 SOCS5。

Long non-coding RNA HCG11 sponging miR-522-3p inhibits the tumorigenesis of non-small cell lung cancer by upregulating SOCS5.

机构信息

Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

Department of Clinical Laboratory, Shandong Maternal and Child Health Care Hospital, Jinan, China.

出版信息

Thorac Cancer. 2020 Oct;11(10):2877-2886. doi: 10.1111/1759-7714.13624. Epub 2020 Aug 26.

BACKGROUND

Numerous studies have shown that long non-coding RNA (lncRNA) is involved in various human diseases including non-small cell lung cancer (NSCLC). The aim of this study was to explore the potential role of lncRNA HCG11 in the pathogenesis of NSCLC.

METHODS

The mRNA expression of HCG11, miR-522-3p and SOCS5 was detected by RT-qPCR. The regulatory mechanism of lncRNA HCG11 was investigated by CCK-8, transwell and dual luciferase reporter assays.

RESULTS

Downregulation of lncRNA HCG11 and upregulation of miR-522-3p were found in NSCLC tissues and cells, and abnormal expressions of lncRNA HCG11 and miR-522-3p were related to adverse clinical outcomes of NSCLC patients. LncRNA HCG11 acted as a molecular sponge for miR-522-3p. Functionally, lncRNA HCG11 inhibited cell viability, migration and invasion in NSCLC by downregulating miR-522-3p. Further, miR-522-3p directly targeted SOCS5. lncRNA HCG11 could positively regulate SOCS5 expression in NSCLC. In addition, HCG11 downregulation or miR-522-3p overexpression abolished the inhibitory effect of SOCS5 on cell viability, migration and invasion in NSCLC.

CONCLUSIONS

LncRNA HCG11 inhibits cell viability, migration and invasion in NSCLC by functioning as a ceRNA of miR-522-3p to upregulate SOCS5.

背景

大量研究表明，长链非编码 RNA（lncRNA）参与包括非小细胞肺癌（NSCLC）在内的多种人类疾病。本研究旨在探讨 lncRNA HCG11 在 NSCLC 发病机制中的潜在作用。

方法

通过 RT-qPCR 检测 HCG11、miR-522-3p 和 SOCS5 的 mRNA 表达。通过 CCK-8、transwell 和双荧光素酶报告基因检测研究 lncRNA HCG11 的调控机制。

结果

在 NSCLC 组织和细胞中发现 lncRNA HCG11 下调和 miR-522-3p 上调，lncRNA HCG11 和 miR-522-3p 的异常表达与 NSCLC 患者的不良临床结局相关。lncRNA HCG11 作为 miR-522-3p 的分子海绵发挥作用。功能上，lncRNA HCG11 通过下调 miR-522-3p 抑制 NSCLC 细胞活力、迁移和侵袭。此外，miR-522-3p 可直接靶向 SOCS5。lncRNA HCG11 可正向调节 NSCLC 中 SOCS5 的表达。此外，HCG11 下调或 miR-522-3p 过表达可消除 SOCS5 对 NSCLC 细胞活力、迁移和侵袭的抑制作用。