通过与原位单噬菌斑杂交筛选λgt重组克隆。
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
作者信息
Benton W D, Davis R W
出版信息
Science. 1977 Apr 8;196(4286):180-2. doi: 10.1126/science.322279.
PMID:322279
Abstract
A rapid, direct method for screening single plaques of Agt recombinant phage is described. The method allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
摘要
本文描述了一种快速、直接筛选λgt重组噬菌体单噬菌斑的方法。该方法每天至少可筛选10⁶个克隆，并简化了重组体的物理隔离。
Zotero 插件