含有黑腹果蝇基因的λ噬菌体重组克隆的筛选及某些特性
Selection and some properties of recombinant clones of lambda bacteriophage containing genes of Drosophila melanogaster.
作者信息
Ilyin Y V, Tchurikov N A
出版信息
Nucleic Acids Res. 1976 Aug;3(8):2115-27. doi: 10.1093/nar/3.8.2115.
Abstract
The lambdagt clones containing fragments of the Drosophila melanogaster genome were prepared and characterized by hybridization of their DNA with (I) lambdagt-cRNA; (2) lambdaC-cRNA; (3) Dm-cRNA; (4) the mRNA of D.melanogaster culture cells and (5) the stable cytoplasmic poly (A) RNA from the same source. The technique for a simple selection of hybrid clones is described. The hybridization with mRNA allows one to select the clones containing structural genes of D.melanogaster. It was found that in all cases when the clone contains the structural gene it also contains the reiterated base sequences of the D.melanogaster genome. Several clones containing D. melanogaster DNA fragments with a size of (2-4)x1O6 daltons hybridizing with a relatively large portion of mRNA were selected for further analysis.
摘要
制备了含有黑腹果蝇基因组片段的λgt克隆，并通过其DNA与以下物质杂交进行表征：（1）λgt - cRNA；（2）λC - cRNA；（3）Dm - cRNA；（4）黑腹果蝇培养细胞的mRNA；（5）来自同一来源的稳定细胞质多聚（A）RNA。描述了一种简单选择杂交克隆的技术。与mRNA杂交可以选择含有黑腹果蝇结构基因的克隆。发现所有情况下，当克隆包含结构基因时，它也包含黑腹果蝇基因组的重复碱基序列。选择了几个含有大小为（2 - 4）×10⁶道尔顿的黑腹果蝇DNA片段且与相对大部分mRNA杂交的克隆进行进一步分析。
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