College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, People's Republic of China.
Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, People's Republic of China.
ACS Appl Mater Interfaces. 2020 Apr 22;12(16):18225-18234. doi: 10.1021/acsami.9b22120. Epub 2020 Apr 10.
Commercial or clinical tissue adhesives are currently limited due to their weak bonding strength on wet biological tissue surface, low biological compatibility, and slow adhesion formation. Although catechol-modified hyaluronic acid (HA) adhesives are developed, they suffer from limitations: insufficient adhesiveness and overfast degradation, attributed to low substitution of catechol groups. In this study, we demonstrate a simple and efficient strategy to prepare mussel-inspired HA hydrogel adhesives with improved degree of substitution of catechol groups. Because of the significantly increased grafting ratio of catechol groups, dopamine-conjugated dialdehyde-HA (DAHA) hydrogels exhibit excellent tissue adhesion performance (i.e., adhesive strength of 90.0 ± 6.7 kPa), which are significantly higher than those found in dopamine-conjugated HA hydrogels (∼10 kPa), photo-cross-linkable HA hydrogels (∼13 kPa), or commercially available fibrin glues (2-40 kPa). At the same time, their maximum adhesion energy is 384.6 ± 26.0 J m, which also is 40-400-fold, 2-40-fold, and ∼8-fold higher than those of the mussel-based adhesive, cyanoacrylate, and fibrin glues, respectively. Moreover, the hydrogels can gel rapidly within 60 s and have a tunable degradation suitable for tissue regeneration. Together with their cytocompatibility and good cell adhesion, they are promising materials as new biological adhesives.
商业或临床用组织粘合剂目前受到限制，因为它们在湿润的生物组织表面的结合强度较弱、生物兼容性低且黏附形成速度较慢。尽管已经开发了儿茶酚改性透明质酸 (HA) 粘合剂，但它们存在局限性：由于儿茶酚基团的取代度低，粘合性不足且降解过快。在这项研究中，我们展示了一种简单有效的策略，可制备具有改进的儿茶酚基团取代度的贻贝启发式 HA 水凝胶粘合剂。由于儿茶酚基团的接枝率显著增加，多巴胺共轭二醛 -HA（DAHA）水凝胶表现出优异的组织黏附性能（即，黏附强度为 90.0 ± 6.7 kPa），明显高于多巴胺共轭 HA 水凝胶（约 10 kPa）、光交联 HA 水凝胶（约 13 kPa）或市售纤维蛋白胶（2-40 kPa）。同时，其最大黏附能为 384.6 ± 26.0 J m，分别是贻贝基粘合剂、氰基丙烯酸酯和纤维蛋白胶的 40-400 倍、2-40 倍和约 8 倍。此外，水凝胶可以在 60 秒内快速凝胶，且具有适合组织再生的可调节降解性。由于其细胞相容性和良好的细胞黏附性，它们有望成为新型生物粘合剂。
