Li Xuedi, Jarosz Alicia Caroline, El-Sohemy Ahmed, Badawi Alaa
Department of Nutritional Sciences, University of Toronto, Canada.
Public Health Risk Sciences Division, Public Health Agency of Canada, Canada.
Nutr Health. 2020 Jun;26(2):151-159. doi: 10.1177/0260106020912945. Epub 2020 Mar 31.
Genetic and nutritional factors play an important role in inflammatory response and diseases. CXCL10 is a critical biomarker that is involved in multiple inflammatory diseases, and elevated levels of CXCL10 have been associated with the development of several chronic and infectious diseases. In contrast, micronutrients can attenuate inflammatory responses. Single nucleotide polymorphisms in the pro-inflammatory cytokine genes such as at rs16944 contributed to a number of inflammatory disorders and may substantiate the convergance between chronic and infectious diseases.
This study aims to identify the modifying effect of nutritional factors on the association between genotypes and CXCL10 levels.
Participants ( = 386) were healthy males and females from the Toronto Nutrigenomics and Health study recruited from the University of Toronto. Levels of micronutrients and inflammatory markers were measured in plasma. genotypes were extracted from the Affymetrix 6.0 SNP chip.
CXCL10 levels were not different across different genotypes. Among those with the GA genotype, elevated CXCL10 levels were observed with higher than median ascorbic acid (β = 0.004 ± 0.002, = 0.047) or higher than median vitamin D status (β = 0.003 ± 0.002, 0.044). Among participants with the AA genotype, subjects with low α-tocopherol status had elevated levels of CXCL10 (β = -0.016 ± 0.007, = 0.012).
The association between rs16944 genotype and CXCL10 levels was modified by the levels of ascorbic acid, α-tocopherol and vitamin D. These findings may aid in understanding the combined effect of genetic and dietary factors in the development of various infectious and chronic diseases in which and CXCL10 may play an etiological role.
遗传和营养因素在炎症反应及疾病中起重要作用。CXCL10是一种关键生物标志物，参与多种炎症性疾病，CXCL10水平升高与多种慢性和感染性疾病的发生有关。相比之下，微量营养素可减轻炎症反应。促炎细胞因子基因中的单核苷酸多态性，如rs16944，与多种炎症性疾病有关，可能证实慢性疾病和感染性疾病之间存在共同之处。
本研究旨在确定营养因素对基因型与CXCL10水平之间关联的修饰作用。
参与者（n = 386）为来自多伦多大学招募的多伦多营养基因组学与健康研究中的健康男性和女性。测量血浆中的微量营养素和炎症标志物水平。从Affymetrix 6.0 SNP芯片中提取基因型。
不同基因型的CXCL10水平无差异。在GA基因型者中，当抗坏血酸高于中位数（β = 0.004±0.002，P = 0.047）或维生素D状态高于中位数时（β = 0.003±0.002，P = 0.044），观察到CXCL10水平升高。在AA基因型参与者中，α-生育酚水平低的受试者CXCL10水平升高（β = -0.016±0.007，P = 0.012）。
抗坏血酸、α-生育酚和维生素D水平修饰了rs16944基因型与CXCL10水平之间的关联。这些发现可能有助于理解遗传和饮食因素在各种感染性和慢性疾病发生中的联合作用，其中rs16944和CXCL10可能起病因学作用。
