S100 钙结合蛋白 A12 的下调抑制神经胶质瘤细胞的生长。
Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells.
机构信息
Department of Neurosurgery, The First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang City, Liaoning Province, P.R. China, 110001.
Department of Neurosurgery, Anshan Hospital of the First Hospital of China Medical University, No.166,Minzhu Street,Tiexi District, Anshan, Liaoning Province, P.R. China, 110001.
出版信息
BMC Cancer. 2020 Mar 30;20(1):261. doi: 10.1186/s12885-020-06768-7.
BACKGROUND
S100 calcium binding protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified.
METHODS
In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of S100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by shRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT, colony formation assay, transwell assay,flow cytometry assa and western blot.
RESULTS
We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT.
CONCLUSION
S100A12 plays a vital role in glioma progression, and may be an important regulatory molecule for biological behaviors of glioma cell lines.
背景
S100 钙结合蛋白 A12（S100A12）是 S100 蛋白家族的一员，广泛表达于中性粒细胞，低表达于淋巴细胞和单核细胞。然而，S100A12 在神经胶质瘤中的作用尚未确定。
方法
本研究通过免疫组织化学方法检测了 81 例神经胶质瘤组织中 S100A12 的表达，以确定 S100A12 在神经胶质瘤细胞中的表达，并评估 S100A12 在神经胶质瘤患者中的临床意义。进一步通过 shRNA 敲低 S100A12，通过 MTT、集落形成实验、Transwell 实验、流式细胞术和 Western blot 评估细胞增殖、细胞迁移和细胞凋亡。
结果
我们发现 S100A12 在神经胶质瘤患者的组织中上调，其表达与 WHO 分级和肿瘤大小相关。进一步的研究发现，敲低 S100A12 通过调节细胞凋亡和 EMT 抑制神经胶质瘤细胞的增殖、迁移和侵袭。
结论
S100A12 在神经胶质瘤的进展中起着重要作用，可能是神经胶质瘤细胞系生物学行为的重要调节分子。
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