雄激素结合球蛋白敲低抑制胶质瘤细胞系的生长。
Androglobin knockdown inhibits growth of glioma cell lines.
作者信息
Huang Bo, Lu Yi-Sheng, Li Xia, Zhu Zhi-Chuan, Li Kui, Liu Ji-Wei, Zheng Jing, Hu Ze-Lan
机构信息
The 2nd Department of Orthopaedics, 117 Hospital of PLA Hangzhou, China.
Yi Ji Shan Hospital of Wan Nan Medical College Anhui, China.
出版信息
Int J Clin Exp Pathol. 2014 Apr 15;7(5):2179-84. eCollection 2014.
Abstract
Globin family was famous for oxygen supply function of its members such as hemoglobin and myoglobin. With the progress of research, several members of this protein family have been proven to play roles in tumors including glioma. Androglobin (ADGB) is a recently identified member of globin family with very few studies about its function. In the present study, we show that ADGB plays an oncogene role in glioma. Lentiviral vector mediated ADGB knockdown inhibited the proliferation of glioma cell lines determined by MTT assay and colony formation assay. ADGB knockdown also increased the apoptosis of glioma cell line U251 assessed by flow cytometry. In addition, western blot showed that ADGB knockdown altered levels of several proteins related to proliferation, survival or apoptosis in U251 cells. These findings suggest ADGB is involved in the progression of glioma in vitro.
摘要
珠蛋白家族以其成员如血红蛋白和肌红蛋白的氧气供应功能而闻名。随着研究的进展，该蛋白质家族的几个成员已被证明在包括胶质瘤在内的肿瘤中发挥作用。雄激素结合球蛋白（ADGB）是珠蛋白家族中最近发现的成员，对其功能的研究很少。在本研究中，我们表明ADGB在胶质瘤中起癌基因作用。慢病毒载体介导的ADGB敲低抑制了通过MTT法和集落形成试验测定的胶质瘤细胞系的增殖。通过流式细胞术评估，ADGB敲低还增加了胶质瘤细胞系U251的凋亡。此外，蛋白质印迹显示ADGB敲低改变了U251细胞中几种与增殖、存活或凋亡相关的蛋白质水平。这些发现表明ADGB在体外参与了胶质瘤的进展。
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本文引用的文献
1
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Int J Oncol. 2014 Feb;44(2):514-20. doi: 10.3892/ijo.2013.2186. Epub 2013 Nov 22.
2
[Current situation and future prospect of cytoglobin research].[细胞珠蛋白研究的现状与未来展望]
Nihon Rinsho. 2013 May;71(5):927-35.
3
The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients.作为预后因素的细胞血红素蛋白在神经胶质瘤中的表达：88 例患者的回顾性分析。
BMC Cancer. 2013 May 20;13:247. doi: 10.1186/1471-2407-13-247.
4
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Hum Mol Genet. 2013 Aug 15;22(16):3207-17. doi: 10.1093/hmg/ddt174. Epub 2013 Apr 15.
5
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Mol Pharmacol. 2013 May;83(5):1109-19. doi: 10.1124/mol.112.083634. Epub 2013 Mar 11.
6
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7
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