The Carter Center, Atlanta, Georgia.
The Carter Center, Kampala, Uganda.
Am J Trop Med Hyg. 2020 Jun;102(6):1411-1416. doi: 10.4269/ajtmh.20-0064.
Uganda has verified elimination of seven onchocerciasis foci since 2007 when the nationwide onchocerciasis elimination policy was launched. However, the Victoria Nile focus (which was eliminated in the early 1970s) had not been verified. The objective of this study was to verify this focus to the WHO verification guidelines and bring it in line with recently eliminated foci. Vector control with dichlorodiphenyltrichloroethane was the main intervention used at the Victoria Nile from the 1950s to the 1970s. Historical fly collection sites along River Nile were identified for recent fly collection. Relevant health workers near the sites were trained to supervise fly collection activity. With support from communities, fly collectors were identified, trained, and equipped to collect flies for at least a year. A total of 854 flies were collected and analyzed by polymerase chain reaction to detect DNA. The communities and their leaders provided consent for the collection of dry blood spots (DBS) from children younger than 10 years for investigation of recent exposure to the disease. A total of 2,953 DBS were collected and analyzed by OV16 ELISA to detect the presence of IgG antibodies recognizing the OV16 antigen. The results showed that none of the flies carried DNA. Similarly, all the children were OV16 negative, showing no exposure to onchocerciasis. All the flies collected were identified as , which is not a known vector for The results confirmed that onchocerciasis and its vector had been eliminated in the Victoria Nile focus.
乌干达自 2007 年启动全国消灭盘尾丝虫病政策以来，已确认消灭了七个盘尾丝虫病流行区。然而，维多利亚尼罗流行区（该流行区于 20 世纪 70 年代初被消灭）尚未得到证实。本研究的目的是根据世界卫生组织的验证准则对该流行区进行验证，并使其与最近消灭的流行区保持一致。20 世纪 50 年代至 70 年代，维多利亚尼罗流行区主要采用滴滴涕进行媒介控制。确定了尼罗河畔历史上的采蝇点，以便进行近期采蝇采集。对附近的相关卫生工作者进行培训，以监督采蝇采集活动。在社区的支持下，确定、培训和装备了采蝇者，以便至少进行一年的采蝇收集。共采集了 854 只苍蝇，并通过聚合酶链反应进行分析，以检测 DNA。社区及其领导人同意从 10 岁以下的儿童中采集干血斑（DBS），以调查近期是否接触过该病。共采集了 2953 份 DBS，并通过 OV16 ELISA 进行分析，以检测是否存在识别 OV16 抗原的 IgG 抗体。结果显示，没有一只苍蝇携带 DNA。同样，所有儿童的 OV16 均为阴性，表明未接触过盘尾丝虫病。所有采集到的苍蝇均被鉴定为 ，这不是已知的 传播媒介。结果证实，维多利亚尼罗流行区的盘尾丝虫病及其传播媒介已被消灭。
